FAMILY CORYNEBACTERIACEAE 



389 



Gelatin: Grows poorly if at all. No 

 liquefaction. 



Agar: Small punctiform colonies. 



Agar slants : Raised, grayish-white, and 

 dry growth (Jones and Little i. Others 

 say cream-colored and moist. 



Blood seruni slants : Fine graj^ puncti- 

 form colonies in 24 hours at 37°C which 

 are a little larger than on agar. Streak 

 scarcely 1 mm in width. Glistening and 

 slimy in fresh cultures. Xo liquefac- 

 tion. 



Litmus milk: Reduction and coagula- 

 tion from the bottom. Slow digestion, 

 becoming alkaline. 



Broth : Sediment at end of 2 days with 

 clear bouillon above. 



Potato: Growth grayish-white; later, 

 becoming a dingy yellow, turning the 

 potato brow^n . 



Acid from glucose. No acid from lac- 

 tose, sucrose, maltose and mannitol. 

 Some strains ferment fructose and man- 

 nose (Merchant. Jour. Bact., 30, 1935, 

 109). 



Shows a close serological relationship 

 with Corynebacterium pseudotuhcrcidosis 

 (Merchant). Anaerobic. 



Not pathogenic for laboratory animals. 

 No toxin produced. 



Optimum temperature 37°C. 



Source : Found in pyelonephritis in 

 cattle. 



Habitat: Occurs in purulent infections 

 of the urinary tract in cattle, sheep, 

 horses and dogs. 



9. Corynebacteritim pseudotuberculo- 

 sis (Buclianan) Eberson. (Nocard, Bull, 

 de la Soc. Centr. de med. Vet., 1885, 207 ; 

 Pseudotuberculose-Bakterien , Preisz , 

 Cent, f . Bakt., 70, 1891, oQS; Bacillus pseu- 

 dotuberculosis ovis Lehmann and Neu- 

 mann, Bakt. Diag., 1 Aufl., 2, 1896, 362; 

 Bacillus pseudotuberculosis Buchiinan, 

 Veter. Bact., Phila., 1911, 238; not 

 Bacillus pseudotuberc^dosis Eisenberg, 

 Bakt. Diag., 3 Aufl., 1891, 294; Eberson, 

 Jour. Inf. Dis., 23, 1918, 10; Corynebac- 

 terium ovis Bergey et al., Manual, 1st 

 ed., 1923, 388; not Corynebacterium pseu- 



dotubercidosis Bergey et al., Manual, 2nd 

 ed., 1925, 394; Corynebacteriimi pseudo- 

 tuberculosis bovis (an evident typographi- 

 cal error) Thomson and Thomson, Ann. 

 Pickett-Thomson Res. Lab., 2, 1926, 132; 

 Corynebacterium pseudolubercidosis ovis 

 Hauduroy et al.. Diet. d. Bact. Path., 

 1937, 159; Corynebacterium preisz -nocardi 

 Hauduroy et al . , ibid., 159 .) From Greek 

 pseudus, a falsehood; Latin tuberculum, a 

 small nodule; M.L., false tuberculosis. 



Common name : Preisz -Nocard bacillus. 



Slender rods: 0.5 to 0.6 by 1.0 to 3.0 

 microns, staining irregularly and showing 

 clubbed forms. Non-motile. Gram- 

 positive. 



Gelatin colonies: Slight development. 



Gelatin stab : No liquefaction. 



Agar colonies : Thin, cream-colored to 

 orange, folded, serrate, dry. 



Loeffler's blood serum: Small, yellow, 

 serrate colonies. No liciuefaction. 



Broth: No turbidity. Granular sedi- 

 ment. Pellicle formed (Carne, Jour. 

 Path, and Bact., 49, 19-39, 316). 



Litmus milk : Unchanged. 



Potato : No growth. 



Nitrites not produced from nitrates. 



Acid from glucose, fructose, galactose, 

 mannose, sucrose, lactose, maltose and 

 de.xtrin. Some strains attack xylose. 



Causes caseous lymphadenitis in sheep 

 and ulcerative lymphangitis in horses. 

 Forms an exotoxin. 



Shows a close serological relationship 

 with Corynebacterium renale (Mer- 

 chant, Jour. Bact., 30, 1935, 109). 



Aerobic, facultative. 



Optimum temperature 37°C. 



Source : From necrotic areas in the 

 kidney of a sheep. 



Habitat: Found in caseous lymphade- 

 nitis in sheep and ulcerative lesions in 

 horses, cattle and other animals. 



10. Corynebacterium kutscheri (Mi- 

 gula) Bergey et al. {Bacillus pseudo- 

 lubercidosis murium Kutscher, Ztschr. 

 f. Hyg., 18, 1894, 338; Bacillus pseudo- 

 tuberculosis murium Lehmann and 

 Neumann, Bakt. Diag., 1 Aufl., 2, 1896, 



