390 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



362; Bacterium kutscheri Migula, Syst. 

 d. Bakt., 2, 1900, 372; Mycobacterium 

 pseudotuberculosis Chester, Manual De- 

 term. Bact., 1901, 355; Conjnebacterium 

 murium Bergey et al., Manual, 1st ed., 

 1923, 386 ; Bergey et al.. Manual, 2nd ed., 

 1925, 395.) Named for the bacteriolo- 

 gist Kutscher, who first isolated the 

 species. 



Rods with pointed ends, staining irreg- 

 ularly. Non-motile. Gram-positive. 



Gelatin colonies: Small, white, trans- 

 lucent. 



Gelatin stab: No growth on surface. 

 White, filiform growth in stab. No 

 liquefaction. 



Agar colonies: Small, thin, yellowish- 

 white, translucent, serrate. 



Agar slant : Thin, white, translucent 



Loeffler's blood serum: Abundant 

 growth. Not peptonized. 



Broth: Slight turbidity. Crystals of 

 ammonium magnesium phosphate are 

 formed. 



Litmus milk : Unchanged. 



Potato : No growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 37°C. 



Source : From cheesy mass in lung of 

 mouse. 



11. Corynebacterium murisepticmn 

 V. Holzliausen. (Cent. f. Bakt., I Abt., 

 Orig., 105, 1927-28, 94.) From Latin 

 TOUs, muris, a mouse; Greek septicus, 

 putrefying, septic. 



Slender rods: 1.2 to 1.5 microns in 

 length, with polar granules. Grow out 

 into long filaments. Non-motile. 

 Gram-positive. 



Gelatin stab : Feeble growth, with 

 fimbriate outgrowth along line of punc- 

 ture. 



Egg glycerol broth : Good growth. 



Loeffler's blood serum : Good growth. 



Broth: Turbid. 



Litmus milk: Acid. No coagulation. 



Potato : Good growth. 



Indole not formed. 



Nitrates not reported. 



Acid from glucose, fructose, galactose, 

 maltose, lactose, sucrose, inulin and 

 mannitol. Arabinose and isodulcitol are 

 not attacked. 



Hydrogen sulfide formed. 



Pathogenic for mice. 



Aerobic, facultative. 



Optimum temperature 37 °C. 



Habitat : Septicemia in mice. 



12. Corynebacterium bovis Bergey et 

 al. (B. pseudodiphtheria, Bergey, The 

 Source and Nature of Bacteria in Milk. 

 Penn. Dept. Agr. Bull. 125, 1904, 11; 

 Bergey et al.. Manual, 1st ed., 1923, 388.) 

 From Latin bos, bovis, ox; of cattle. 



Rods, slender, barred, clubbed, 0.5 to 

 0.7 by 2.5 to 3.0 microns. Non-motile. 

 Gram-positive. 



Gelatin stab: Slight, gray, flat surface 

 growth . 



Agar colonies : Circular, gray, slightly 

 raised, radiate, undulate, dry. 



Agar slant: Thin, gray, filiform, dry 

 growth. 



Broth: Slight granular sediment. 



Litmus milk: Slowly becoming deeply 

 alkaline. 



Potato : No growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



No acid from cai'bohydrate media. 



Blood serum: Thin, gray, filiform 

 growth. 



Causes rancidity in cream. Weakly 

 lipolytic on tributyrin agar (Black, Jour. 

 Bact., 4i, 1941, 99). 



Optimum temperature 37 °C. 



Source : In fresh milk drawn directly 

 from the cow's udder. 



Note : Miss Alice Evans (personal com- 

 munication) states that the organism 

 from the udder which she described as 

 Bacterium lipoUjticus (sic) {Bacillus 

 abortus var. lipolyticus Evans, Jour. 

 Inf. Dis., 18, 1916, 459; Bacterium abor- 

 tus var. lipolyticus Evans, Jour. Bact.,^, 



