FAMILY CORYNEBACTERIACEAE 



391 



1917, 185; Evans, Jour. Inf. Dis., 22, 191S, 

 576; not Bacterium lipolyticum Huss, 

 Cent. f. Bakt., II Abt., 20, 1908, 474; 

 Alcaligenes lipolyticus Pacheco, Revista 

 da Sociedade Paulista de Med. Vet., 3, 



1933, 9) was probably a Corynehacterium. 

 This is also regarded as probable by 

 Steck (Die latente Infektion der Alilch- 

 driise, Hanover, 1930) and by Bendixen 

 (Ztschr. f. Infektionskrankh. d. Haus- 

 tier., 43, 1933, 106). Miss Evans also 

 indicates that it is probable that the 

 organism described by Bergey first in 

 1904 {loc. cit.) and later in the first 

 edition of the Manual as Corynehacterium 

 bovis was the same organism. This is 

 further confirmed by Black (Jour. Bact., 

 41, 1941, 99). A description of Bacte- 

 rium lipolyticum Evans will be found in 

 the Manual, 5th ed., 1939, 803. 



13. Corynebacterium equlMagnussen. 

 (^Nlagnusson, Arch. f. Tierheilk. 50, 

 22; Corynebacterium pyogenes {equi) 

 Meissner and Wetzel, Deutsche Tier- 

 arztl. Wchnschr., 31, 1923, 449; Coryne- 

 bacterium (pyogenes) equi roseum Liitji, 

 ibid., 561; Mycobacterium equi Jensen, 

 Proc. Linn. Soc. New So. Wales, 59, 



1934, 33; Corynebacterium magnusson- 

 holth Plum, Cornell Vet., 30, 1940, 15; 

 Corynebacterium purulenlus Holtman, 

 Jour. Bact., 49, 1945, 161.) From Latin 

 equus, horse. 



Description from Dimock and Ed- 

 wards, Kentucky Agri. Exper. Stat., 

 Bull. 333, 1932; Bruner and Edwards, 

 ibid., Bull. 414, 1941; Merchant, Jour. 

 Bact., 30, 1935, 95; and Brooks and 

 Hucker, Jour. Bact., 48, 1944, 309. 



Rods variable according to medium. 

 Coccoid and ellipsoidal cells to rather 

 long curved and sometimes clubbed 

 forms. The latter are especially apt to 

 occur in liquid media. Non-motile. 

 Gram-positive. 



Gelatin stab : Good growth. No lique- 

 faction. 



Agar colonies : Usually moist, smooth 

 and glistening, tan to yellow (Brooks 



and Hucker, loc. cit., p. 300) or pink to 

 red chromogenesis (Merchant, loc cit 

 p. 107). 



Agar slant : Moist heavy growth which 

 may run down the slant (Dimock and 

 Edwards, loc. cit., p. 322). 



Broth: Turbid with no pellicle and 

 little sediment (Dimock and Edwards, 

 loc. cit., p. 322). Pellicle and final pH 

 alkaline (Brooks and Hucker, loc. cit., 

 p. 309). Branched cells occur in 6 to 8 

 hour cultures in broth. 



Loeffler's blood serima: Good growth 

 with tan to yellow chromogenesis. No 

 liquefaction. 



Coagulated egg yolk: Vigorous salmon- 

 pink growth. Dryer than on agar, re- 

 sembling wrinkled growth of tubercle 

 bacillus after two weeks. 



Litmus milk: No change to slightly 

 alkaline. 



Potato : Abundant growth, usually tan, 

 j^ellow or pink. 



Indole not formed. 



Hydrogen sulfide produced on appro- 

 priate media. 



Nitrites produced from nitrates. No 

 ammonia produced. 



No acid from carbohydrate media. 

 However, glucose stimulates growth. 



Sodium hippurate: Not hydrolyzed. 



Esculin: Not hydrolyzed. 



No exotoxin demonstrated in filtrate 

 of broth cultures. 



No or slight hemolysis of horse blood. 



Not pathogenic for laboratory animals. 



Aerobic. 



Temperatui-e relations: Optimum 25° 

 to 37 °C. Maximum 37° to 45 °C. Mini- 

 mum 7° to 18°C. 



Source: Originally isolated from in- 

 fectious pneumonia of foals. 



Habitat : Found in spontaneous pneu- 

 monia of foals and other infections in 

 horses. Also in swine, cattle and 

 buffaloes. 



Note: Jensen (loc. cit., 33) regards 

 four cultures of soil bacteria isolated in 

 Australia as identical with this organism. 



