392 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Because of the acid -fast staining of the 

 cells, especially when grown in milk for 

 3 to 7 days, he places this species in the 

 genus Mycobacterium. Most cocci re- 

 tain the stain completely, while the rods 

 take the counterstain. Jensen thinks 

 the organism a widespread soil saprophyte 

 which under certain conditions acquires 

 pathogenic properties. He points out 

 the close relationship of this organism to 

 Bacterium aurantium-roseum Honig 

 (Mededeel . Deli Proefstat. te Medan, 7, 

 1912, 223) isolated from fermenting 

 tobacco. He also regards this species as 

 closely related to Mycobacterium coel- 

 iacum Gray and Thornton. Red strains 

 seem to be much like Bacillus rubroper- 

 tinctus Hefferan and Micrococcus (Sta- 

 phylococcus) erythromyxa Zopf. 



14. Corynebacterium paurometabo- 

 lum Steinhaus. (Jour. Bact., 4^ , 1941, 

 763 and 783.) From Greek paurus, 

 little; metabole, change or little action. 



Rods : 0.5 to 0.8 by 1.0 to 2.5 microns, 

 occurring singly, in pairs and in masses. 

 Metachromatic granules present. Non- 

 motile. Gram-positive. 



Gelatin stab : Slow liquefaction at sur- 

 face. 



Agar colonies : White to gray, entire, 

 circular, small, dry, somewhat granular. 



Agar slant : Filiform to arborescent, 

 thick, granular growth. 



Broth : Abundant granular sediment 

 but no turbidity. Pellicle. 



Litmus milk: Alkaline. 



Potato : Thick, raised, dry, granular, 

 profuse, gray to light cream-colored 

 growth. 



Indole not produced. 



Slight production of hydrogen sulfide. 



Nitrites not produced from nitrates. 



No action on the following carbohy- 

 drates : Glucose, lactose, sucrose, maltose, 

 fructose, mannitol, galactose, arabinose, 

 xylose, dextrin, salicin, raffinose, tre- 



halose, sorbitol, inulin, dulcitol, glycerol, 

 rhamnose, adonitol, mannose, esculin 

 and inositol. 



Aerobic. 



Slight alpha hemolysis. 



Non-pathogenic for guinea pigs. 



A special semi-solid medium, the main 

 nutritive constituents of which were pro- 

 teose peptone, rabbit serum, gelatin, 

 minced rabbit kidney and carbohydrates, 

 was used for the original isolation. An 

 incubation period of 4 to 7 days at 26°C 

 was necessary for the initial isolation. 

 Subsequent transfers to ordinary beef- 

 infusion agar grew out in 24 to 48 hours. 



Source : From media inoculated with 

 the mycetome and ovaries of the bedbug, 

 Cimex lectularius L. A very similar 

 diphtheroid strain was isolated from the 

 alimentary tract of the bagworm, Thyri- 

 dopteryx ephemeraejormis Haw. 



Habitat : Distribution in nature un- 

 known. 



*15. Corynebacterium insidiosum (Mc- 

 culloch) Jensen. (Aplanobacter insi- 

 diosum McCulloch, Phytopath., 15, 1925, 

 497 ; Bacteriimi insidiosum Stapp, in 

 Sorauer, Handb. der Pflanzenkr., 2, 5 

 Aufl., 1928, 178; Phytomonas insidiosa 

 Bergey et al.. Manual, 3rd ed., 1930, 278; 

 .Jensen, Proc. Linnean Soc. of New So. 

 Wales, 59, 1934, 41.) From Latin in- 

 sidiosus, deceitful, dangerous. 



Also see McCulloch, Jour. Agr. Res., 

 33,1926,502. 



Rods: 0.4 to 0.5 by 0.7 to 1.0 micron. 

 Capsules present. Non-motile. Gram- 

 positive. 



Gelatin: Slow liquefaction. 



Beef agar colonies : Pale yellow, circu- 

 lar, smooth, shining ; edges entire ; viscid. 

 Blue granules found on the medium. 



Milk: Coagulated after 16 to 20 days. 

 No digestion. An apricot yellow sedi- 

 ment is deposited on the walls of the 

 tube. 



* Descriptions of Species nos. 15 to 20 inclusive prepared by Professor Walter H. 

 Burkholder, Ithaca, New York. 



