FAMILY ACHROMOBACTERIACEAE 



415 



Nitrite production from nitrates vari- 

 able. 



Starch not hydrolyzed. 

 Blood serum not liquefied. 

 No action on carbohydrates. 

 Aerobic, facultative. 

 Optimum temperature 22"C. 

 Habitat: Intestinal canal. 



4. Alcaligenes bookeri (Ford) Bergey 

 et al. (Bacillus A of Booker, Trans. 

 Ninth Internat. Med. Congress, 3, 1887, 

 598; Bacillus bookeri Ford, Studies from 

 the Royal Victoria Hospital, Montreal, 1, 

 1903, 31; Bergey et al.. Manual, 1st ed., 

 1923, 236; Bacterium bookeri Levine and 

 Soppeland, Eng. Exp. Sta., Iowa State 

 College, Bui. 77, 1926, 55.) Named for 

 the bacteriologist who first isolated this 

 species. 



Rods: 0.5 by 1.5 to 2.0 microns, occur- 

 ring singly. Motile with peritrichous 

 flagella. Gram-negative. 



Gelatin colonies: Circular, brown, 

 variable in size. 



Gelatin stab: Slow, saccate liquefac- 

 tion, becoming stratiform. 



Agar colonies: Thin, transparent, with 

 opaque center and indistinct margin. 



Agar slant : Abundant, yellowish to yel- 

 lowish-brown . 



Broth: Turbid, with viscid sediment. 

 No pellicle. 



Litmus milk: Alkaline. Soft curd. 

 Litmus reduced. Peptonization. 



Potato: Luxuriant, yellowish-white, 

 moist. Medium is darkened. 



Indole not formed. 



Nitrites not produced from nitrates. 



No acid or gas from carbohydrate 

 media. 



Blood serum : Yellowish-brown growth. 

 Gradual liquefaction. 



No characteristic odor. 



Aerobic, facultative. 



Optimum temperature 37 °C. 



Source : From alvine discharges of chil- 

 dren suffering with cholera infantum. 



Habitat: Intestinal canal. 



5. Alcaligenes recti (Ford) Bergey et 

 al. {Bacterium recti Ford, Studies from 

 the Royal Victoria Hospital, Montreal, 

 1, 1903, 31 ; Bergey etal., Manual, 1st ed., 

 1923, 236.) From Latin rectus, rectum. 



Rods: 0.5 by 1.5 to 2.0 microns, occur- 

 ring singly, in pairs and in chains. Mo- 

 tile with peritrichous flagella. Gram- 

 negative. 



Gelatin colonies : Variable in size and 

 shape, circular to oval, brown. 



Gelatin stab: Rapid, saccate liciuefac- 

 tion. 



Agar colonies: Large, grayish-white, 

 with opaque center. Slightly spreading. 



Agar slant: Grayish-white, echinulate. 



Broth: Turbid. No pellicle. 



Litmus milk : Alkaline. No peptoniza- 

 tion. 



Potato: Luxuriant, moist, brownish- 

 red. 



Indole not formed. 



Nitrites produced from nitrates. 



No acid or gas from carbohydrate media. 



Blood serum: Abundant white growth. 

 No liquefaction. 



No characteristic odor. 



Aerobic, facultative. 



Optimum temperature 37°C. 



Source : Found but once from coecum 

 and rectum (Ford). 



Habitat : Intestinal canal. 



6. Alcaligenes marshallii Bergey et al. 

 (Bacillus B of Marshall, Cent. f. Bakt., 

 II Abt., 11, 1903, 739; Bacterium lactis 

 marshalli Conn, Esten and Stocking, Ann. 

 Rept. Storrs Agr. Exp. Station, 1906, 141 ; 

 Bergey et al., Manual, 1st ed., 1923, 237.) 

 Named for Prof. C. E. Marshall, the 

 American bacteriologist who first isolated 

 this species. 



Rods: 0.3 by 1.2 microns, occurring 

 singly. Non-motile. Gram-negative. 



Gelatin colonies: Gray, granular, ir- 

 regular, glistening. 



Gelatin stab: Slow, infundibuliform 

 liquefaction. 



Agar slant: Filiform, gray to creamy- 

 white, raised, becoming lemon-yellow. 



