FAMILY ACHROMOBACTERIACEAE 



419 



Nitrites produced from nitrates. 

 Starch hydrolyzed. 



Acid from glucose and sucrose. Oc- 

 casionally from maltose and glycerol. 

 Attacks phenol and naphthalene. 

 Aerobic, facultative. 

 Optimum temperature 30° to 35°C. 

 Source : Fifteen cultures from soil. 

 Habitat : Soil . 



4. Achromobacter delicatulum (Jor- 

 dan) Bergey et al. {Bacillus delicatulus 

 Jordan, Report Mass. State Bd. of 

 Health, 1890, 837; Bacterium delicatulus 

 Chester, Ann. Rept. Del. Col. Agr. Exp. 

 Sta., 9, 1897, 82; Bergey et al., Manual, 

 Isted., 1923, 137.) FromLatin delicatus, 

 soft, delicate; M. L. delicatulus, some- 

 what delicate. 



Characters added to Jordan's descrip- 

 tion by Bergey {loc. cit.) from his private 

 notes are indicated. Steinhaus (Jour. 

 Bact., J^, 1941, 771) apparently found the 

 same organism and has added other 

 characters. 



Rods: 1.0 by 2.0 microns, occurring 

 singly (Jordan). Motile, possessing peri- 

 trichous flagella. Gram-negative (Ber- 

 gey)- 



Gelatin colonies: Whitish, homogene- 

 ous, with radiate margin. 



Gelatin stab : Infundibuliform lique- 

 faction. 



Agar slant: Whitish, glistening. 



Broth: Turbid, with gray pellicle and 

 sediment. 



Litmus milk: Acid. Slow reduction 

 and peptonization (Steinhaus). 



Potato: Thin, gray streak. 



Acid from glucose, sucrose, maltose and 

 lactose (slow) (Steinhaus). 



No hydrolysis of starch (Steinhaus). 



No HjS produced (Steinhaus). 



Indole not formed (Bergey). 



Nitrites produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 30° to 35°C. 



Source : From the effluent of a septic 

 tank (Jordan). From water (Bergey). 



From the alimentary tract of an adult 

 Colorado potato beetle (Leptinotarsa 

 decemlineata Say) (Steinhaus) . 



Habitat : Presumably widely distrib- 

 uted in nature. 



5. Achromobacter aquamarinus ZoBell 

 and Upham. (Bull. Scripps Inst, of 

 Oceanography, Univ. Calif., 5, 1944, 264.) 

 From Latin aqua, water, and marinus, 

 sea. 



Rods: 0.8 by 1.2 to 2.0 microns, with 

 rounded ends, occurring singly. Motile 

 by means of a few peritrichous flagella. 

 Gram-negative. 



All media except the fresh-water broth, 

 litmus milk, and potato were prepared 

 with sea water. 



Gelatin colonies: 2 mm, convex, circu- 

 lar, entire, whitish. 



Gelatin stab: Poor growth, no lique- 

 faction, no pigment. 



Agar colonies: 2 mm, convex, smooth, 

 circular. 



Agar slant: Moderate, beaded, glisten- 

 ing, butyrous growth with no pigment. 



Sea-water broth: Surface ring, mod- 

 erate turbidity, heavy viscous sediment. 



Fresh-water broth: Poor growth. 



Litmus milk: No visible change. 

 Casein not digested. 



Potato: No visible growth. 



Indole not formed. 



Nitrites rapidly produced from ni- 

 trates. 



Produces acid but no gas from glucose 

 and maltose. Does not ferment lactose, 

 sucrose, mannitol, glycerol, xylose, or 

 salicin. 



Starch not hydrolyzed. 



Hydrogen sulfide not formed. 



Ammonia produced from peptone but 

 not from urea. 



Fats are hydrolyzed. 



Aerobic, facultative. 



Optimum temperature 20° to 25°C. 



Source : Found in sea water and on sub- 

 merged slides. 



Habitat : Sea water. 



