422 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Gelatin stab : A bluish -gray growth 

 occurs along the line of inoculation. 

 No liquefaction. 



Glucose agar colonies: Bluish-gray, 

 circular, smooth, glistening, entire. 



Broth: Uniform turbidity with viscid 

 sediment. 



Litmus milk: Unchanged. 



Acid from glucose but little or no 

 action on other carbohydrates. 



Potato: Slight, grayish growth. 



Aerobic, facultative. 



Innocuous when fed to bees. Not 

 pathogenic when inoculated subcutane- 

 ously in rabbits. 



Source : Occurs as a secondary invader 

 in European foulbrood of bees. 



Habitat: Unknown. 



12. Achromobacter delmarvae Smart. 

 (Smart, Jour. Bact., 23, 1932, 41 and 

 Jour. Agr. Research, 51, 1935, 363.) 

 From Delmarva, coined from Del., Mar. 

 and Va., the regions in which the species 

 was found. 



Short rods: Average size 0.75 by 1.5 

 microns, with rounded ends, occurring 

 singly, in pairs and in short chains. Non- 

 motile. Gram-negative. 



Gelatin colonies : Similar to agar 

 colonies. 



Gelatin stab: Scanty growth. No 

 liquefaction. 



Beef-infusion agar colonies : Small, cir- 

 cular, raised, edges smooth, glistening, 

 translucent, bluish-white, amorphous, 

 margin entire. 



Agar stab : Abundant growth. Sur- 

 face growth round, smooth, glistening, 

 bluish-white, raised. Filiform growth 

 the whole length of stab, but growth 

 best at top. 



Agar slant: Abundant filiform growth, 

 raised, glistening, smooth, tran.slucent, 

 bluish-white, no odor; old cultures 

 slightly viscid. Medium unchanged. 



Nutrient broth: Turbid. Delicate 

 white pellicle. Sediment abundant, 

 white, slightly stringy. No odor. Color 

 of medium unchanged. 



Sterile milk: Slow growth. No 

 peptonization. Coagulation in 12 to 

 14 days. Milk turns chocolate brown 

 beginning at top. 



Litmus milk: Acid with reduction of 

 litmus in 5 days. Coagulation with 

 return of pink color in 12 to 14 days. 

 Browning of medium. 



Potato: Abundant growth, grayish- 

 white, glistening, smooth, raised. Me- 

 dium changed from white to smoke -gray. 



Indole not formed. 



Nitrites produced from nitrates in 7 

 days at 26°C. 



No HaS produced. 



Ammonia not formed. 



Diastatic action weak. 



Acid but no gas from glucose, lactose, 

 glycerol and mannitol. Alkaline reac- 

 tion and no gas from sucrose. 



Optimum pH 7.0. 



Temperature relations : Optimum 

 26 °C. Good growth up to 31 °C. Very 

 slight growth at 37° and at -8°C. 



Facultative anaerobe. 



Source : Isolated from fresh straw- 

 berries from Delaware, Maryland and 

 Virginia. 



Habitat : Unknown. 



Appendix: Many of the following 

 species were described before Gram 

 and fiagella stains had been perfected. 

 Hence it is impossible to identify them 

 definitely as belonging to Achromobacter. 

 Comparative study is needed in other 

 cases before the remaining species can be 

 placed in their proper place in the genus. 



Achromobacter acidum (Chester) 

 Bergey et al. (Species No. 56 of Conn, 

 Storrs Agr. Exper. Sta., 7th Ann. Rept. 

 for 1894, 1895, 83; Bacterium acidum 

 Chester, Man. Determ. Bact., 1901, 

 146; Bergey et al., Manual, 1st ed., 1923, 

 151.) From milk. See Manual, 4th 

 ed., 1934, 246 for a description of this 

 organism. 



Achromobacter agile (Ampola and 

 Garino) Bergey et al. (Bacilhis denitri- 

 ficans agilis Ampola and Garino, Cent. 



