432 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Oceanography, Univ. Calif., 5, 1944, 278.) 

 From Greek hals, salt and hydror, water. 



Short rods: 0.6 by 0.8 to 1.0 microns, 

 occurring singly. Motile by means of 

 many peritrichous flagella. Gram-nega- 

 tive. 



All media except the fresh-water broth, 

 litmus milk, and potato were prepared 

 with sea water. 



Gelatin colonies: Small, circular, 

 orange. 



Gelatin stab: Napiform liquefaction 

 becoming crateriform. Beaded along line 

 of stab. 



Agar colonies: 2 mm, pulvinate, circu- 

 lar, entire, smooth. 



Agar slant : Moderate, glistening, echin- 

 ulate, butyrous growth with yellow pig- 

 ment. 



Sea-water broth: Yellow surface ring, 

 heavy turbidity, moderate viscid sedi- 

 ment. 



Fresh -water broth: No visible growth. 



Litmus milk: No visible change. 

 Casein not digested. 



Very poorly tolerant of increases or de- 

 creases in salinity. 



Potato: No visible growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Produces acid but no gas from glucose, 

 lactose, maltose, sucrose, and salicin. 

 Does not ferment glycerol, mannitol, or 

 xylose. 



Starch is hydrolyzed. 



Hydrogen sulfide not formed. 



Ammonia produced from peptone but 

 not from urea. 



Fats not hydrolyzed. 



Aerobic, facultative. 



Optimum temperature 20° to 25°C. 



Source : Sea water and marine mud. 



Habitat : Sea water. 



9. Flavobacterium neptunixun ZoBell 

 and Upham. (Bull. Scripps Inst, of 

 Oceanography, Univ. Calif., 5, 1944, 278.) 

 From Latin Neptunius, god of the sea. 



Rods: 0.5 to 0.6 by 1.6 to 4.5 microns, 

 many bent rods, occurring singly and in 



short chains. Motile by means of long, 

 peritrichous flagella. Gram-negative. 



All media except the fresh-water broth, 

 litmus milk, and potato were prepared 

 with sea water. 



Gelatin colonies: Small, circular, 

 darker centers, sink in gelatin, faintly 

 yellow. 



Gelatin stab : Slow napiform liquefac- 

 tion. Filiform growth along line of stab. 



Agar colonies: 2 mm, circular, smooth, 

 entire, convex, dark centers with buff 

 pigment. 



Agar slant : Luxuriant, echinulate, glis- 

 tening, slightly mucoid growth with buff 

 to yellow pigment. Agar discolored 

 brown. 



Sea-water broth: Heavy pellicle, 

 scanty turbidity, scanty sediment. 



Fresh-water broth : No visible growth. 



Litmus milk: No visible change. 

 Casein not digested. 



Potato : No visible growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Produces acid but no gas from glucose, 

 lactose, maltose, and salicin. Does not 

 ferment glycerol, mannitol, xylose, or 

 sucrose. 



Starch is hydrolyzed. 



Hydrogen sulfide not formed. 



Ammonia produced from peptone but 

 not from urea. 



Fats not hydrolyzed. 



Aerobic, facultative. 



Optimum temperature 20° to 25°C. 



Source : Marine bottom deposits. 



Habitat : Sea water. 



10. Flavobacterium suaveolens Soppe- 

 land. (Jour. Agr. Res., 28, 1924, 275.) 

 From Latin siiaveolens, of a sweet odor. 



Rods: 0.6 to 0.8 by 1.0 to 1.2 microns, 

 with rounded ends, occurring singly and 

 in pairs. Motile, with peritrichous fla- 

 gella. Gram-negative on plain agar. 

 Gram-positive in young culture on milk 

 powder agar. 



Gelatin stab: Rapid stratiform lique- 

 faction. Medium becomes brown. 



