FAMILY ACHROMOBACTERIACEAE 



433 



Agar colonies: Small, circular, smooth, 

 yellow, amorphous, undulate margin. 



Agar slant: Moderate, flat, glistening, 

 opaque, butyrous, yellow, with aromatic 

 odor. 



Broth: Turbid with scanty sediment. 

 Aromatic odor, becoming cheesy. 



Litmus milk: Peptonized. Alkaline. 



Potato: Abundant, yellow, glistening, 

 becoming brown. 



Indole formed. 



Nitrites are produced from nitrates. 



Hydrogen sulfide formed. 



Slight acid but no gas from glucose, 

 sucrose and glycerol. No acid from lac- 

 tose . 



Starch hydrolyzed. 



Blood serum is liquefied. 



Aerobic, facultative. 



Optimum temperature 25°C. 



Source : Dairy wastes. 



Habitat: Unknown. 



11. Flavobacterium rhenanus (Migula) 

 Bergey et al. (Rhine water bacillus of 

 Burri, Frankland and Frankland, Micro- 

 organisms in Water, 1894, 483; Bacillus 

 rhenanus Migula, Syst. d. Bakt., 2, 1900, 

 713; Bacillus rheni Chester, Manual De- 

 term. Bact., 1901, 251; Bergey et al.. 

 Manual, 1st ed., 1923, 103.) Named for 

 the Rhine River. 



Characters added to Burri's descrip- 

 tion by Bergey (loc. cit.) from his private 

 notes are indicated. Steinhaus (Jour. 

 Bact., ^2, 1941, 771) apparently found the 

 same organism and has added other char- 

 acters. 



Rods: 0.7 by 2.5 to 3.5 microns, with 

 rounded ends, occurring singly and in 

 chains (Burri). Motile, possessing peri - 

 trichous flagella (Bergey). Gram-nega- 

 tive (Bergey). 



Gelatin colonies: Convex, colorless, 

 transparent, becoming yellowish. 



Gelatin stab: Infundibuliform lique- 

 faction. 



Agar colonies : Small, smooth, convex, 

 entire. 



Glycerol agar slant: Thin, shining, 

 honey-colored. Growth dry and tough. 



Broth: Turbid, with orange-colored 

 pellicle and sediment. 



Litmus milk: Soft coagulum, becoming 

 slightly alkaline with yellow ring. 



Potato: Moist, glistening, thin, flat, 

 orange to rust-colored. 



Indole not formed (Bergey). 



Nitrites produced from nitrates (Ber- 

 gey). 



Acid from glucose, maltose, and su- 

 crose but not lactose (Steinhaus). 



No hydrolysis of starch (Steinhaus). 



No HoS produced (Steinhaus). 



Aerobic facultative. 



Optimum temperature 30°C. 



Source : From Rhine River water 

 (Burri). From water (Bergey). From 

 eggs in ovary of a walking stick 

 (DiapheromerafemorataSsiy) (Steinhaus). 



Habitat : Presumably widely distrib- 

 uted in nature. 



12. Flavobacterium marinum Harri- 

 son. (Canadian Jour, of Research, /, 

 1929, 234.) From Latin marinits, per- 

 taining to the sea. 



Rods: 0.8 by 1.2 to 1.3 microns, with 

 rounded ends. Occur singly and in 

 pairs. Motile with 4 to 5 peritrichous 

 flagella. Encapsulated. Gram-variable. 

 Show blue granules in Gram-negative 

 rods . 



Gelatin colonies : Circular, iridescent, 

 whitish margin with pale yellow center. 

 Liquefaction. 



Gelatin stab : Saccate to stratiform 

 liquefaction. 



Agar colonies: Circular, pale yellow, 

 smooth, convex, granular, reticulate edge. 



Agar slant: Amber-yellow, slightly 

 raised, spreading, smooth, glistening, 

 transparent. 



Ammonium phosphate agar : Scant 

 growth. 



Broth : Turbid, sediment. 



Litmus milk: Alkaline. Digestion 

 without coagulation. Clear serum. 



Potato: Abundant, amber-yellow, be- 

 coming dirty yellow, spreading, glisten- 

 ing. 



