434 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Indole not formed. 



Nitrites not produced from nitrates. 

 Trace of ammonia formed. 



Faint acidity from glucose. No action 

 on lactose or sucrose. 



Loeffler's blood serum not liquefied. 

 Faint yellow spreading gi'owth. 



No H2S formed. 



Aerobic, facultative. 



Optimum temperature 20° to 25°C. 



Source : Isolated from living halibut 

 obtained at 30 to 50 fathoms, Pacific 

 Ocean. Gibbons (Contrib. to Canadian 

 Biol, and Fish., 8, No. 22, 1934, 279) re- 

 ports this species as occurring in the slime 

 and feces of cod (Gadus callarias), hali- 

 but (Ilippoglossus hippogloss^is) and 

 skate {Raja erinacea) . 



Habitat : Skin and feces of fishes. 



13. Flavobacterium harrisonii Bergey 

 et al. (Variety No. 6, Harrison, Rev. 

 gen. du Lait, 5, 1905, 129; Bacillus lactis 

 harrisonii Conn, Esten and Stocking, 

 Ann. Rept. Storrs Agr. Exp. Sta., 1906, 

 169; Bergey et al.. Manual, 1st ed., 1923, 

 104.) Named for Prof. F. C. Harrison, 

 the Canadian bacteriologist who first iso- 

 lated this species. 



Rods : 0.25 to 0.75 by 0.3 to 3.5 microns, 

 occurring singly and occasionally in short 

 chains. Motile, possessing peritrichous 

 flagella. Gram-negative. 



Gelatin colonies: Small, gray, glisten- 

 ing, lobular, citron-yellow, slimy. 



Gelatin stab: Villous growth in stab. 

 Slow crateriform to napiform liquefac- 

 tion. 



Agar slant : Lu.xuriant, viscous, spread- 

 ing, becoming dirt}', to brownish citron- 

 yellow. 



Broth : Turbid, with viscid ring and 

 gelatinous sediment, sweetish odor, al- 

 kaline. 



Litmus milk : Colorless to gray and 

 slimy, becoming yellow, alkaline. 



Potato : Luxuriant, yellow, spreading, 

 slimy. 



Indole not formed. 



Glucose, lactose, maltose and sucrose 

 broth turn alkaline with a disagreeable 



odor. Reaction of glycerol broth remains 

 neutral. 



Aerobic, facultative. 



Optimum temperature 25°C. 



Source : Slimy milk. 



Habitat : Unknown. 



14. Flavobacterium invisibile (Vaughan) 



Bergey et al. (Bacillus invisihilis 

 Vaughan, American Jour. Med. Sci., IO4, 

 1892, 191 ; Bacterium invisibilis Chester, 

 Ann. Rept. Del. Col. Agr. Exp. Sta., 9, 

 1897, 77; Bergey et al.. Manual, 1st ed., 

 1923, 109.) From Latin invisibilis, not 

 visibile. 



Rods: 0.6 to 0.7 by 1.2 to 2.0 microns, 

 occurring singly. Motile, possessing 

 peritrichous flagella. Gram-negative. 



Gelatin colonies : Pale yellow, burr- 

 like, with irregular margin. 



Gelatin stab : Scanty growth on surface. 

 Good growth in stab. No liquefaction. 



Agar colonies: White, convex, smooth, 

 serrate. 



Agar slant : Limited, thick, white 

 streak. 



Broth: Turbid. 



Litmus milk: Unchanged. 



Potato : No growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 35°C. 



Habitat : Water. 



15. Flavobacterium lactis Bergey et al. 

 {Bacillus uruiiiaLicus lactis Grimm, Cent. 

 f. Bakt., II Abt., 8, 1902, 584; Bacillus 

 aromaticus Grimm, ibid., 589; not 

 Bacillus aruinaticus Pammel, Bull. 21, 

 Iowa Agr. Exp. Sta., 1893, 792; Bergey 

 et al., Manual, 1st ed., 1923, 108.) From 

 Latin lac, milk. 



Rods: 0.7 to 1.0 by 3.5 to 4.0 microns, 

 occurring singly, in pairs and in chains. 

 Motile, possessing peritrichous flagella. 

 Gram -negative. 



Gelatin colonies ; Circular, light yellow, 

 slimy. Concentrically ringed, undulate. 



Gelatin stab: Slimy surface growth. 

 No liquefaction. 



