FAMILY ACHROMOBACTERIACEAE 



435 



Agar slant: Slimj-, yellowish, smooth, 

 moist. 



Broth : Turbid, with abundant sedi- 

 ment. 



Litmus milk : Slightly acid. 



Potato : Thick, slimy, brownish, with 

 yellowish margin. 



Indole not formed. 



Nitrites not produced from nitrates. 



Cultures have pleasant odor. 



Aerobic, facultative. 



Optimum temperature 25°C. 



Source : Isolated from milk. 



Habitat : Unknown. 



16. Flavobacterium sewanense (Ka- 

 lantarian and Petrossian) Bergey et al.- 

 (Bacterium sewanense Kalantarian and 

 Petrossian, Cent. f. Bakt., II Abt., 85, 

 1932, 431 ; Bergey et al., IManual, 4th ed., 

 1934, 160.) From M. L., Sevan, a lake in 

 Armenia. 



Straight or curved rods: 1.0 to 2.0 b\' 

 4.0 to 5.0 microns on Molisch's agar; on 

 meat extract agar and potato agar they 

 are short or even coccoid. Ends rounded, 

 occurring singly or in pairs. Non-motile. 

 Gram reaction not given. Presumably 

 negative . 



Gelatin stab : Slow liquefaction. 



Agar colonies: Circular, raised, glisten- 

 ing, dirty white. Deep colonies yellow 

 and lens-shaped. 



Agar slant : Abundant, dirty yellow, 

 glistening, raised. 



Broth : Turbid with characteristic 

 growth forms. Pellicle formed in old cul- 

 tures. 



Milk: Unchanged. 



Potato : Yellow, raised, glistening, with 

 darkening of the medium. 



No visible gas produced from carbo- 

 hydrates. 



Crystals of calcium carbonate form in 

 old cultures on CaCU and Molisch's agar. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source : Isolated from pellicle formed 

 on surface of fish infusions in Lake Sevan 

 and tap waters containing 1 per cent 

 CaCL. 



Habitat : Sea water. Thought to pro- 

 duce deposits of CaCOa in Lake Sevan, 

 S. S. R. Armenia. 



17. Flavobacterium arborescens 



(Frankland and Frankland) Bergey et al. 

 {Bacillus arborescens Frankland and 

 Frankland, Ztschr. f. Hyg., 6, 1889, 379; 

 also see Tils, Ztschr. f. Hyg., 9, 1890, 312; 

 Zimmermann, Bakt. unserer Trink- u. 

 Nutzwasser, 2, 1894, 20; and Wright, 

 Mem. Nat. Acad. Sci., 7, 1894, 446, var. 

 a and b; Bacterium arborescens Chester, 

 Ann. Rept. Del. Col. Agr. Exp. Sta., 9, 

 1897, 106; IMigula, Syst. d. Bakt., 2, 1900, 

 468 uses Bacillus arborescens in the text 

 by mistake as Bacterium is used for other 

 species in the genus and Bacterium ar- 

 borescens is used in the index, p. 1058; 

 not Bacillus arborescens Chester, Man. 

 Determ. Bact., 1901, 249; Erythrobacillus 

 arborescens Holland, Jour. Bact., 5, 1920, 

 217; Bergey et al., Manual, 1st ed., 1923, 

 113.) From Latin arborescens, becoming 

 a tree or tree-like. 



Rods: 0.5 by 2.5 microns, occurring 

 singly and in chains. Non-motile (Frank- 

 lands). Gram-negative (Zimmermann). 



Gelatin colonies : Radiate branching 

 filaments. Center yellowish, border 

 translucent. 



Gelatin stab : Liquefied with yellow 

 deposit. 



Agar slant : Dirty orange growth. 



Broth: Turbid, with orange sediment. 

 No pellicle. 



Litmus milk : Slow coagulation ; litmus 

 reduced. Reaction unchanged (Wright). 



Potato : Deep orange, luxuriant growth. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 30°C. 



May belong to Corynebacterium (Leh- 

 mann and Neumann, Bakt. Diag., 7 Aufl., 

 2, 1927, 709). 



Source : From river and lake water. 



Habitat: Water. 



17a. Bacillus arborescens Chester. 

 (Bacillus arborescens non-liquefaciens 

 Ravenel, Mem. Nat. Acad. Sci., 8, 1896, 



