436 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



39; Bacterium arborescens non-Iique- 

 faciens Chester, Ann. Rept. Del. Col. 

 Agr. Exp. Sta., 9, 1897, 103; not Bac- 

 terium arborescens non-liquefaciens von 

 Rigler, Hyg. Rund., 12, 1902, 479; Ches- 

 ter, Man. Determ. Bact., 1901, 249.) 

 Regarded by author as a non-liquefying 

 strain of Bacillus arborescens Frankland 

 and Frankland. Not a yellow chronio- 

 gen. From soil. 



18. Flavobacterium lutescens (Mig- 

 ula) Bergey et al. (Der gelbe Bacillus, 

 Lustig, Diagnostik der Bakterien des 

 Wassers, 1893, 78; Bacterium lutescens 

 Migula, Syst. d. Bakt., 2, 1900, 476; 

 Bergey et al., Manual, 1st ed., 1923, 114.) 

 From Latin lutum, yellow; lutescens, be- 

 coming yellowish. 



Rods: 0.5 by 0.95 micron, occurring 

 singly and in pairs. Non-motile. Gram- 

 negative. 



Gelatin colonies: Circular, yellow, 

 lobate. 



Gelatin stab : Slow liquefaction. 



Agar slant : Pale yellow, becoming 

 golden yellow. 



Broth: Turbid. 



Litmus milk: Alkaline. 



Potato: Lu.xuriant, golden-yellow 

 growth. 



Indole not formed. 



Nitrites produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 30° to 35°C. 



Source : From water. Gibbons (Con- 

 trib. to Canadian Biol, and Fish., 8, No. 

 22, 1934, 279) reports this species as oc- 

 curring in the slime of the cod (Gadus 

 callarias ) . 



Habitat : Fresh and salt water. 



19. Flavobacterium fucatiun Harrison. 

 (Canadian Jour, of Research, 1, 1929, 

 232.) From Latin /(tea/ us, painted, col- 

 ored. 



Rods: 0.8 to 1.0 by 2.5 to 3.5 microns, 

 slightly bent, with rounded ends. Gran- 

 ular with diphtheroid forms at 37°C. Non- 

 motile. Gram-negative. 



Gelatin colonies : Circular, yellow, en- 

 tire, paler at edges. 



Gelatin stab : Crateriform liquefaction. 



Agar colonies: Circular, buff -yellow, 

 smooth, shiny, convex to pulvinate, gran- 

 ular, entire. 



Agar slant : Moderate, light buff -yellow, 

 spreading, shiny, smooth. 



Ammonium phosphate agar : Good 

 growth in 6 days. 



Broth : Turbid, becoming clear, pellicle 

 and yellow sediment. 



Litmus milk: Alkaline. Peptonized. 

 Clear serum. Yellow sediment. 



Potato : Abundant, pale buff-yellow, 

 smooth, spreading, becoming orange-yel- 

 low. 



Indole not formed. 



Nitrites produced from nitrates. 



Traces of ammonia formed. 



No acid from glucose, lactose or suc- 

 rose. 



Loeffler's blood serum not liquefied. 

 Light buff-yellow growth becoming ochra- 

 ceus salmon. 



No H2S formed. 



Aerobic, facultative. 



Optimum temperature 20° to 25°C. 



Source : Repeatedly isolated from living 

 halibut obtained at 30 to 50 fathoms, 

 Pacific Ocean. Also isolated by Gibbons 

 (Contrib. to Canadian Biol, and Fish., 8, 

 No. 22, 1934, 279) from cod (Gadus 

 callarias) and dogfish {Squalus acan- 

 thias). 



Habitat : Skin of fishes. 



20. Flavobacterium esteroaromaticxmi 

 (Omelianski) Bergey et al. (Bacterium 

 esteroaromaticum Omelianski, Jour. Bact., 

 8, 1923, 407; Bergey et al., Manual, 3rd 

 ed., 1930, 149.) From M. L., ester and 

 Greek aromaticus, aromatic. 



Rods: 0.5 by 1.0 to 3.0 microns. Non- 

 motile. Gram reaction not recorded. 



Gelatin stab: Crateriform liquefaction 

 with odor of musk melons. 



Agar colonies: Circular, yellow-brown, 

 with fimbriate margin and a fruity aroma. 



