462 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Potato : Growth abundant, butyrous, 

 glistening, raised, pinkish. 



Indole not formed. 



Blood is hemolyzed. 



Loeffler's serum: Abundant, glistening 

 growth. Liquefaction. 



No H2S produced. 



Ammonia is produced. 



Although Duran-Reynals and Clausen 

 report nitrites not produced from ni- 

 trates, a retest of their cultures by 

 Breed has shown that nitrites are actively 

 produced from nitrates. 



Acid from glucose, fructose, sucrose, 

 mannitol, maltose, galactose and salicin. 

 Dextrin, lactose, inulin, dulcitol, xylose 

 and arabinose slightly attacked or not at 

 all. 



Pigment production : Water-soluble 

 pigment produced. Pink coloration best 

 shown on glycerol potato. Reddish col- 

 oration best shown in peptone water with 

 2 per cent glucose, the yellow coloration 

 in glucose broth and the black coloration 

 in the sediment of liquefied gelatin and 

 peptone water. Some non-pigmented 

 strains. 



Temperature relations: Grows well at 

 20°C. Growth more abundant at 37°C. 

 Practically no growth at 10°C. Thermal 

 death point 60°C for 20 minutes. 



Aerobic. 



Pathogenicity : Pathogenic for am- 

 phibians, reptiles and to some extent fish. 

 Lesions are produced in the iguanid liz- 

 ards (Anolis equestris and Anolis caroli- 

 nensis), the gekkonid lizards {Tarentola 

 mauritanica and Hemidactylus brookii), 

 the garter snake (Thamnophis butleri) 

 and the brown snake {Storeria dekayi), 

 the musk turtle (Sternothaerus odoratus), 



the toad {Bufo americanus) , the frog 

 (Rana pipiens) and the catfish (Ameiu- 

 ras melas). When the inoculated animal 

 is kept at 37°C, the disease becomes 

 general and usually is fatal. Non-patho- 

 genic for warm-blooded animals (Clausen 

 and Duran-Reynals, Amer. Jour. Path., 

 13, 1937, 441). 



Source : From tumor-like lesions in 

 Cuban lizards (Anolis equestris). Also 

 isolated from iguanid lizards (Basiliscus 

 vittatus) from Mexico by Clausen and 

 Duran-Reynals {loc. cit.). 



Habitat: The cause of a natural, non- 

 fatal, contagious disease of lizards. 



3. Salmonella sp. (Type Arizona). 

 {Salmonella sp., Dar es salaam Type var. 

 from Arizona, Caldwell and Ryerson, 

 Jour. Inf. Dis., 65, 1939, 245; Salmonella 

 arizona Kauffmann, Acta Path, et Micro- 

 biol. Scand., 17, 1940, or 19, 1942; Ari- 

 zona culture, Edwards, Cherry and Bru- 

 ner, Jour. Inf. Dis., 73, 1943, 236; Sal- 

 monella arizona Group, Edwards, Jour. 

 Bact., 49, 1945, 513.) 



Ferments lactose and liquefies gelatin. 

 Antigenic structure: XXXIII: Z4, Z23, 

 zae: — . 



Source: Isolated by Caldwell and Ry- 

 erson (loc. cit.) from horned lizards, 

 Gila monsters and chuckawallas. Also 

 pathogenic for guinea pigs and rabbits. 

 Found in snakes by Hinshaw and 

 McNeill (Cornell Vet., 34, 1944, 248). 

 Also reported by Edwards (loc. cit.) 

 from infants. 



Habitat: Apparently widely distri- 

 buted in lizards, snakes, and warm- 

 blooded animals. 



