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MANUAL OF DETERMINATIVE BACTERIOLOGY 



Broth: Turbid, moderate sediment 

 and delicate pellicle in old cultures. 



Litmus milk: Slight, transient acidity, 

 followed by a return to neutral or to 

 slight alkalinit}^ 



Potato: Delicate, moist, slightly 

 spreading, barely visible growth. 



Acid but no gas from glucose, fructose, 

 galactose, xylose, maltose, raffinose, 

 dextrin, glycerol, mannitol and sorbitol. 

 No action on lactose, sucrose, inulin, 

 rhamnose, inositol, salicin and usually 

 arabinose and dulcitol. 



Reduces trimethylamine oxide (Wood 

 and Baird, loc. cit.). 



Indole not formed. 



No characteristic odor. 



Nitrites produced from nitrates. 



Hydrogen sulfide produced. 



Aerobic, facultative. 



Optimum temperature 37°C. 



Antigenic structure: IX, XII, [Vi]: 

 d : — . The somatic antigens are related 

 to those of Salmonella enteritidis and a 

 number of other species of Salmonella. 

 V and W forms are present (Felix and 

 Pitt, Jour. Path, and Bact., 38, 1934, 

 409; Craigie and Brandon, Jour. Path, 

 and Bact., 43, 1936, 233 and 239). 

 Craigie and Yen (Canadian Public 

 Health Journal. 29, 1938, 448 and 484) 

 by the action of selected Vi phages recog- 

 nize eleven distinct stable types of 

 Salmonella typhosa which have been 

 found to be of epidemiological impor- 

 tance. 



Source: From the human intestine. 



Habitat: The cause of typhoid fever. 

 Pathogenic for laboratory animals on 

 parenteral injection. Isolated once 

 from a chicken by Henning, Onderste- 

 poort, So. Africa. 



Note : This species has previously 

 been regarded as the type species of the 

 genus Eberthella Buchanan (Acystia 

 Enderlein, Sitzber. Gesell. Naturf. 

 Freunde, Berlin, 1917, 317; Buchanan, 

 Jour. Bact., 3, 1918, 53; Eberthus Cas- 

 tellani and Chalmers, Man. Trop. Med., 



3rd ed., 1919, 934; Lankoides Castellani 

 and Chalmers, ibid., 938; Wesenbergus 

 Castellani and Chalmers, ibid., 940.) 



67. Salmonella enteritidis (Gaertner) 

 Castellani and Chalmers. {Bacillus en- 

 teritidis Gaertner, Correspond, d. AUge- 

 mein. Artzl. Verein Thuringen, 17, 1888, 

 573; Klebsiella enteritidis De Toni and 

 Trevisan, in Saccardo, Sylloge Fun- 

 gorum, 8, 1889, 923; Bacterium enteritidis 

 Chester, Ann. Rept. Del. Col. Agr. Exp. 

 Sta., 9, 1897, 68; Bacillus gaertner 

 Morgan, Brit. Med. Jour., 1, 1905, 1257; 

 Castellani and Chalmers, Manual Trop. 

 Med., 3rd ed., 1919, 930.) Named for the 

 disease, enteritis. 



Rods: 0.6 to 0.7 by 2.0 to 3.0 microns, 

 occurring singly, in pairs and occasion- 

 ally in short chains. Motile with peri- 

 trichous flagella. Gram-negative. 



Gelatin colonies: Circular, gray, trans- 

 lucent, granular, entire. 



Gelatin stab: Abundant surface 

 growth. No liquefaction. 



Agar colonies: Circular, gray, trans- 

 lucent, moist, smooth, entire. Desko- 

 witz and Buchbinder (Jour. Bact., 29, 

 1935, 294) describe a variant that pro- 

 duces a soluble yellow pigment where 

 certain peptone is present in the agar. 

 Antigenic structure not determined. 



Agar slant: Grayish-white, opalescent, 

 smooth, moist, undulate growth. 



Broth: Turbid, with thin pellicle and 

 grayish-white sediment. 



Litmus milk: Slightly acid, becoming 

 alkaline, opalescent, translucent to yel- 

 lowish-gray. 



Potato: Al)undant, moist, yellowish- 

 brown to brown growth. 



Indole not formed. 



Nitrites produced from nitrates. 



Acid and gas from glucose, fructose, 

 galactose, mannose, arabinose, xylose, 

 maltose, trehalose, dextrin, glycerol, 

 mannitol, dulcitol and sorbitol. No acid 

 or gas from lactose, sucrose, inulin, 

 salicin, raffinose, adonitol and inositol. 



