550 



MANUAL OF DETERMIXATIVE BACTERIOLOGY 



and quickly by P. pseudotuberculosis; 

 same for methylene blue, Janus green 

 and thionin. No growth on Bessonowa 

 media (pH 5.9). See Yersinia, p. 703. 



4. Pasteurella pseudotuberculosis 



(Eisenberg) Topley and Wilson. (Ba- 

 cillus der pseudotuberculose, Ffeiffer, 

 Ueber die bacillare Pseudotuberculose bei 

 Nagetieren, Leipzig, 1889 ; Bacillus pseu- 

 dotuberculosis Eisenberg, Bakt. Diag., 

 3 Aufl., 1891, 294; Streptobacillus pseudo- 

 tuberculosis rodentium Preisz, Ann. Inst. 

 Past., 8, 1894, 231; Bacterium pseudo- 

 tuberculosis rodentium Lehmann and 

 Neumann, Bakt. Diag., 1 Aufl., 2, 1896, 

 362; Bacillus pseudotuberculosis roden- 

 tium Lehmann and Neumann, ibid., 429; 

 Bacterium pseudotuberculosis Migula, 

 Syst. d. Bakt., 2, 1900, 374; Corynebac- 

 terium rodentium Bergey et al.. Manual, 

 1st ed., 1923, 386; Corynebacterium 

 pseudotuberculosis Bergey et al., Manual, 

 2nd ed., 1925, 394; Topley and Wilson, 

 Princip. Bact. and Immun., 1st ed., 2, 

 1931, 825; Corynebacterium pseudotu- 

 berculosis rodentium Kelser, IVIan. Vet. 

 Bact., 2nd ed., 1933, 319; Malleomyces 

 pseudotuberculosis rodentium Pribram, 

 Klassifikation d. Schizomyceten, 1933, 

 93.) Latinized, false tuberculosis. 



Bacillus tuberculosis zoogloeicae Malas- 

 sez and Vignal (Ann. de Physiol., 1883, 

 370) is considered identical with this 

 species by Hauduroy et al. (Diet. d. 

 Bact. Path., 1937, 162). 



The original tabular description by 

 Eisenberg, Bakt. Diag., 3 Aufl., 1891, 294 

 is very incomplete. Description taken 

 from Topley and Wilson, Princip. Bact. 

 and Immun., 2nd ed., 1936, 607 and 

 Bessonowa, Lenskaja and Molodtzowa, 

 Office Internat. d'Hyg. Publ., 29, 1937, 

 2106. 



Small rods : Variable in size and shape. 

 Ellipsoidal or coccoid forms 0.8 by 0.8 

 to 2.0 microns, with rounded ends, occur- 

 ring singly. Rod-shaped forms 0.6 by 

 1.5 to 5.0 microns, with rounded ends, 

 occurring singly, in groups or in short 

 chains. Occasionally long curved fila- 



ments. Motile (Weitzenberg, Cent. f. 

 Bakt., I Abt., Orig., 133, 1935, 343). 

 Non-acid-fast. Gram-negative. 



Gelatin stab : After 7 days at 22°C, good 

 filiform growth extending to bottom of 

 tube. No liquefaction. 



Agar colonies: After 24 hours at 37°C, 

 circular, 0.5 to 1.0 mm in diameter, 

 umbonate, granular, translucent, gray- 

 ish-yellow, butyrous; edge entire; dull, 

 finely granular or beaten-copper surface ; 

 differentiated into a raised, more opaque 

 center and a flat, clearer periphery with 

 radial striation. 



Agar slant: After 48 hours at 37°C, 

 growth moderate, confluent, raised, gray- 

 ish-yellow, translucent, with glistening, 

 wavy or boaten-copper surface and an 

 irregularl}^ lobate edge. 



Blood agar plate : Good growth. No 

 hemolysis. 



Broth : After 24 hours at 37°C, mod- 

 erate growth with moderate turbidity 

 which later clears. Viscous sediment. 

 Incomplete surface and ring growth. 

 Becomes alkaline more rapidly than 

 Pasteurella pestis. 



Potato : After 7 days at 22°C, a thin 

 yellowish membrane which later turns 

 brown. 



Indole not formed. 



Litmus milk : Usually slightly alkaline. 



Nitrites produced from nitrates. 



Ammonia is produced. 



Acid but no gas from glucose, maltose, 

 mannitol, salicin, arabinose, xylose, 

 rhamnose and glj^cerol. Sometimes acid 

 from sucrose. 



Hydrogen sulfide produced. 



Catalase positive. 



Methyl red positive. 



Methylene blue is reduced. 



Voges-Proskauer test negative. 



Temperature relations : Optimum 30°C. 

 Minimum 5°C. Maximum 43 °C. Ther- 

 mal death point 60°C for ten minutes. 



Pathogenicity : The cause of sponta- 

 neous disease in rabbits, rats and guinea 

 pigs. Infectious for mice, rats, dogs, 

 cats and horses. 



Aerobic, facultative. 



