556 



MANUAL OF DETERMINATIVIO BACTERIOLOGY 



35, 1933, 44; LoeJJlerella whitmori Gay 

 et al., Agents of Disease and Host Re- 

 sistance, Indianapolis, 1935, 791 ; Breed, 

 in Manual, 5th cd., 1939, 300.) Latin- 

 ized, false glanders. 



Short rods : With rounded ends, occur- 

 ring singly and in short chains, showing 

 bipolar staining. Motile. Gram-nega- 

 tive. 



Gelatin stab : Moderate, crateriform 

 liquefaction. 



Agar colonies : Circular, slightly raised, 

 thick, opaque, cream-colored with ir- 

 regular margin. 



Glycerol agar slant : Wrinkled, thick, 

 rugose, cream-colored growth. 



Broth: Turbid with pellicle. 



Litmus milk: Curdling with slowly 

 developed acidity, pink sediment ; may 

 be digested. 



Potato: Vigorous, cream-colored 

 growth. 



Indole not formed. 



Acid from glucose, maltose, lactose, 

 sucrose and mannitol. 



Blood serum slowly liquefied. 



Aerobic, facultative. 



Optimum temperature 37°C. 



Source : Lesions and blood in rats, 

 guinea pigs, rabbits and man. Once 



from a transient nasal discharge in a 

 horse (Stanton, Fletcher and Symonds) 

 and once from a splenic abscess in a cow 

 (Nicholls). 



Habitat: Glanders-like infection (meli- 

 oidosis) in rats, guinea pigs, rabbits and 

 in man in India, Federated Malay States 

 and Indo-China. 



Appendix: The following may belong 

 in this genus : 



Flavobacierium orchitidis Sherwood, 

 Irwin and Marts. (Sherwood, Irwin 

 (not Edwin) and Marts, Amer. Jour. 

 Diseases of Children, 45, 1933, 446; 

 Sherwood, Jour. Kansas Med. Soc, 34, 

 1933, 220.) From a case of meningitis. 

 Sherwood (personal communication, 

 1945) now considers this organism iden- 

 tical with Bacillus whitmori (MaUeo- 

 myccs pseudomallei). See Manual, 5th 

 cd., 1939, 53S for a description of this 

 species. 



Malleomyces agliaceus Pribram. 

 (Bacillo opale agliaceo, Vincenzi, Giorn. 

 d. R. Accad. d. Med. Torino, 1890, No. 

 6; Pribram, Klassification der Schizomy- 

 ceten, Leipzig, 1933, 93.) Cause of 

 ))seudotuberculosis in frogs. 



Genus III. Actinobacillus Brumpt* 

 (Precis de Parasitologic, Paris, 1st ed., 1910, 849.) 



Medium-sized, aerobic, Gram-negative rods which frequently show much pleomor- 

 phism. Coccus-like forms frequent. Tendency to bipolar staining. Acid but no gas 

 produced from carbohydrates. Grow best, especially when freshly isolated, under 

 increased CO2 tension. Pathogenic for animals; some species attack man. The out- 

 standing characteristic of the group is the tendency to form aggregates in tissues or 

 culture which resemble the so-called sulfur granules of actinomycosis. 



The type species is Actinobacillus lignieresi Brumpt. 



1. Actinobacillus lignieresi Brumpt. 

 (Actinobacilo, Lignieres and Spitz, Bole- 

 tin d. Agri. y Ganaderia, Buenos Aires, 

 11, 1902, 169; Actinobacillus, Lignieres 



and Spitz, Cent. f. Bakt., I Abt., Orig., 

 35, 1903, 294; Brumpt, Precis de Para- 

 sitol., Paris, 1st ed., 1910, 849; Bacillus 

 lignieri (sic) Mace, Traite de Bact^riolo- 



* Revised by Prof. W. A. Hagan, New York State Veterinary College, Ithaca, New 

 York, December, 1938; further revision, December, 1945. 



