558 



MANUAL OF DETERMIXATIVE BACTERIOLOGY 



Aerobic, facultative. 



Distinctive character : Manner of 

 growth in liquid gelatin. 



Source : Found in lesions of actinomy- 

 cosis. 



Habitat : Presumably in actinomycotic 

 lesions. 



3. Actinobacillus actinoides (Smith) 

 Topley and Wilson. ( Bacillus actinoides 

 Th. Smith, Jour. Exp. Med., 28, 1918, 

 333; Actinomyces actinoides Bergey et al.. 

 Manual, 1st ed., 1923, 346; Topley and 

 Wilson, Princip. of Bact. and Immun., 

 1st ed., 1, 1931, 256.) From Greek, ray- 

 like. 



Slender rods in tissues. In cultures 

 may be bacillary or coccoid in form. 

 Grows only under increased CO-, tension 

 (so-called microaerophilic). Does not 

 grow on ordinary agar or broth, except 

 occasionally when transferred from more 

 favorable media. Most characteristic 

 growth on coagulated blood serum. 



Gelatin: No growth. 



Agar colonies : Very minute, pale, straw 

 color. 



Agar slant : Best growth seen in water 

 of condensatioia. Serial transfers on 

 this medium generally fail. 



Broth: No growth. 



Litmus milk: No growth. 



Potato: No growth. 



Coagulated blood serum (cow) : Growth 

 appears first in the condensation water. 

 Appear as granules, consisting of cap- 

 sular material in which bacillary forms 

 are embedded. Surface mulberry-like 

 because of club-like extensions of capsu- 

 lar material. In stained preparations, 

 the capsular material appears amorphous. 



Optimum temperature 37 °C. 



Microaerophilic. 



Not pathogenic for laboratory animals, 

 except possibly the white rat in which a 

 spontaneous chronic pneumonia occurs 

 caused by an organism indistinguishable 

 from this one. Experiments with rats 

 by artificial inoculation have not been 

 reported. 



Source : From lungs of calves suffering 

 from chronic pneumonia. 



Habitat : Has not been recognized in 

 nature except in pathological processes. 



