FAMILY parvobactp:riaceae 



559 



* APPENDIX TO TRIBE PASTEURELLEAE. 



While the authors who describe the following new genus with its single species do 

 not indicate its general relationships, it would appear to be as closely related to the 

 species placed in Parrobacteriaccae as to those in anj' other family. It is therefore 

 placed in this appendix pending a clarification of the situation. 



Genus A. Donovania Anderson, De Monbreun and Goodpasture. 



(Jour. Exp. Med., 81, 1945, 25.) Named for C. Donovan who first described the 

 type species. 



Pleomorphic non-motile rods, exhibiting single or bipolar condensations of 

 chromatin. Occur singly and in clusters. May be capsulated or non-capsulated. 

 Gram -negative. Growth outside human body occurs only in the yolk, yolk sac or 

 amniotic fluid of developing chick embryo or in a medium containing embryonic yolk. 

 Pathogenic for man causing granulomatous lesions, particularly in the inguinal region. 



The type species is Donovania granulomatis Anderson, De Monbreun and Good- 

 pasture . 



1. Donovania granulomatis Anderson 

 et al. (Epithelial cell parasites, Dono- 

 van, Indian Med. Gaz., Jfi, 1905, 414; 

 Donovan bodies, Dienst, Greenblatt and 

 Sanderson, Jour. Inf. Dis., 62, 1938, 112; 

 Donovan organism, Anderson, Science, 

 97, 1943, 560; Anderson, De Monbreun 

 and Goodpasture, Jour. Exp. Med., 81, 

 1945, 25.) From M. L. granuloma, of 

 granuloma. 



Pleomorphic rods 1 to 2 microns in 

 length, with i-ounded ends, occurring 

 singly and in clusters. Intracellular 

 forms usually capsulated. Non-motile. 

 Gram-negative. 



No growth on ordinary culture media. 



Chick embryo : Grows readilj^ in yolk, 



yolk sac and feebly in amniotic fluid of 

 developing chick embryo. 



Embryonic yolk medium : Growth 

 occurs. 



Distinctive characters : Capsulated 

 forms readily demonstrated by means of 

 Wright's stain as blue bacillary bodies 

 surrounded by well-defined dense pink- 

 ish capsules. Non-capsulated forms 

 variable in morphology. Characteristic 

 safety-pin forms may be demonstrated. 



Not pathogenic for the common experi- 

 mental animals. 



Source : Granulomatous lesions of man. 



Habitat : Human lesions. The cause 

 of granuloma inguinale. 



* Prepared by Dr. Orren D. Chapman, Syracuse Medical College, Syracuse, New 

 York, March, 1946. 



