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MANUAL OF DETERMINATIVE BACTERIOLOGY 



TRIBE II. BRUCELLEAE BERGEY, BREED AND MURRAY. 



(Preprint, Manual, 5th ed., October, 1938, vi.) 



Small, motile or non-motile rods or coccoids which grow on special media. There 

 is a single genus Brucella. 



Genus I. Brucella Meyer and Shaw* 



(Jour. Inf. Dis., 27 , 1920, 173.) Named for Sir David Bruce, who first recognized 

 the organism causing undulant fever. 



Short rods with many coccoid cells, 0.5 by 0.5 to 2.0 microns ; non-motile ; capsulated ; 

 Gram-negative; gelatin not liquefied; neither acid nor gas from carbohydrates; urea 

 utilized; parasitic, invading all animal tissues, producing infection of the genital 

 organs, the mammary gland, the respiratory and intestinal tracts; pathogenic for 

 various species of domestic animals and man. 



The type species is Brucella melitensis (Hughes) Meyer and Shaw. 



Key to the species of genus Brucella. 



I. Non-motile. 



A. Grow in special media containing basic fuchsin. 



1. Grows in media containing thionin. 



1. Brucella melitensis. 



2. Does not grow in media containing thionin. 



2. Brucella abortus. 



B. Does not grow in media containing basic fuchsin. 

 1. Grows in media containing thionin. 



3. Brucella sui's. 

 II. Motile. 



4. Brucella bronchiseptica. 



Differential characters of the three closely related species of genus Brucella. 



* All utilize glucose in shake cultures. 



1. Brucella melitensis (Hughes) 

 Meyer and Shaw. (Bruce, Practitioner, 

 S9, 1887, 161; ibid., 40, 1888, 241; Kept. 

 Army Med. Dept., London, 82, 1890, 



Append. No. 4, 465; streptococcus Mile- 

 tensis (sic) Hughes, The Mediterranean 

 Naturalist, 2, February 1, 1892, 325; 

 Micrococcus melitensis Bruce, Ann. Inst. 



* Revised by Prof. I. F. Huddleson, Michigan State College, East Lansing, Michigan, 

 December, 1942. 



