562 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



1914, 86 (30); authorship established by 

 Traum in North Amer. Vet., 1, No. 2, 

 1920; described as Bacillus abortus by 

 Good and Smith, Jour. Bact., 1, 1916, 

 415; Huddleson, Undulant Fever Sym- 

 posium, Amer. Pub. Health Assoc, 

 (Oct., 1928) 1929, 24; also Mich. Agr. 

 Exp. Sta. Tech. Bull. 100, 1929, 12; 

 Brucella melitensis var. suis Hardy, 

 Jordan, Borts and Hardy, Public Health 

 Reports, 4S, 1930, 2433; Bacillus abortus 

 suis Meyer, Amer. Jour. Pub. Health, 

 21, 1931, 503.) From Latin, of swine. 



The morphological and cultural charac- 

 ters are similar to those of Brucella 

 melitensis. 



S cultures of Brucella suis can be dif- 

 ferentiated from S cultures of Brucella 

 melitensis, but not from S cultures of 

 Brucella abortus, by the agglutinin 

 absorption test. 



Source : From urinogenital and many 

 other organs of swine. 



Habitat : Chief host the hog. Causes 

 abortion in swine and undulant fever 

 (brucellosis) in man. Also infectious 

 for horses, dogs, cows, monkeys and 

 laboratory animals. 



The ditferentiation of the above species 

 of Brucella by the bacteriostatic action 

 of dyes depends upon the medium used. 

 When tryptose agar (Difco) is used, 

 basic fuchsin and thionin should be used 

 in a final dilution of 1:100,000. 



There are several forms of the R and 

 mucoid phases of Brucella spp. (Huddle- 

 son, Amer. Jour. Vet. Res., 7, 1946, 5). 

 The true R type differs from the S type 

 in its lack of pathogenicity, its antigenic 

 properties, its susceptibility to aggluti- 

 nation by exposure of suspensions to 

 heat and to basic dyes in concentration 

 of 1 : 2000, and colonial appearance. The 

 mucoid phases differ antigenically, mor- 

 phologically and culturally. Colonies 

 on agar are spherical or flat, regular in 

 contour, grayish to mucoid in appear- 

 ance. Suspensions are not agglutinated 

 by heat or dyes, or always by special 



agglutinating serums. There is no 

 change in their growth characteristics 

 on media containing either basic fuchsin 

 or thionin. 



4. Brucella bronchiseptica (Ferry) 

 Topley and Wilson. (Ferry, Amer. Vet. 

 Rev., 37, 1910, 499; also see McGowan, 

 Jour. Path., 15, 1911, 372; Bacillus bron- 

 chicanis Ferry, Jour. Inf. Dis., 8, 1911, 

 402; Bacillus bronchisepticus Ferry, 

 Amer. Vet. Rev., 41, 1912, 79; Bacterium 

 bronchisepticus Evans, Jour. Inf. Dis., 

 18, 1916, 578; Bacterium bronchicanis 

 Holland, Jour. Bact., 5, 1920, 221 ; Alcali- 

 genes bronchisepticus Bergey et al., 

 Manual, 1st ed., 1923, 234; Topley and 

 Wilson, Princip. Bact. and Immun., 

 1st ed., 1, 1931, 508.) Latinized, disease 

 of the bronchial tubes. 



Evans {loc. cit., 593) regards Bacterium 

 bronchisepticus as related to Bacterium 

 abortus morphologically, culturally, bio- 

 chemically and serologically. 



Short slender rods: 0.4 to 0.5 by 2.0 

 microns, usually occurring singly, some- 

 times in pairs and chains. Motile with 

 4 to 6 peritrichous flagella (Topley and 

 Wilson). Gram-negative. 



Gelatin colonies : Similar to those on 

 agar. 



Gelatin stab : Slow filiform growth. 

 No liquefaction. 



Agar colonies: Small, opaque, white, 

 slightly raised, porcellaneous, entire. 



Agar slant : Growth moderate but more 

 luxuriant than in Brucella melitensis, 

 filiform, slightly raised, smooth, opales- 

 cent, lustrous, moist, entire. 



Broth: Turbid, with thin, gray pellicle 

 and ropy sediment. Musty odor de- 

 velops. 



Litmus milk: Alkaline. No coagula- 

 tion. 



Potato : Growth fairly abundant, 

 brownish, glistening, moist, sticky. Me- 

 dium is darkened. 



Indole not formed. 



Nitrites often produced from nitrates 

 (Topley and Wilson). 



