570 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Bact., 25, 1933, 401; Pasteurella vulgata 

 Pr^vot, Ann. Inst. Past., 60, 1938, 292.) 

 From Latin vulgatus, common. 



Oval rods : 0.7 to 2.5 microns long, usu- 

 ally occurring singly, sometimes in 

 pairs. One strain formed filaments 10 

 microns long. Stain solidlj'', some 

 strains show bipolar staining. Morphol- 

 ogy very variable in glucose broth. 

 Non-motile. Gram-negative. 



Gelatin: Liquefied in 4 to 20 days by 

 all but one strain. 



Blood agar colonies: Soft, translucent, 

 grayish, elevated, 1.5 to 2.0 mm in diam- 

 eter. Half of the strains are hemolytic. 



Broth: Heavy and diffuse growth. 



Indole not formed. 



Hydrogen sulfide is formed. 



Milk : Acidified. Coagulated by some 

 strains in 5 to 25 days. 



Nitrites not produced from nitrates. 



Acid and a small amount of gas from 

 arabinose, dextrin, fructose, galactose, 

 glucose, glycogen, inulin, lactose, maltose, 

 mannose, raffinose, rhamnose, starch, 

 sucrose and xylose. Seven strains 

 fermented esculin. No acid or gas from 

 amygdalin, cellobiose, glycerol, mannitol, 

 melezitose, salicin, sorbitol, trehalose, 

 dulcitol, erythritol or inositol. 



Non-pathogenic for white mice and 

 rabbits. 



Anaerobic. 



Distinctive characters: Does not form 

 indole; does not produce gas from pep- 

 tone. This is the commonest species 

 found in the feces of adults. Differs 

 from Bacteroides incormnunis in that 

 it does not ferment amygdalin and cello- 

 biose, but does ferment glycogen and 

 starch. Liquefies gelatin. 



Source : Thirty-eight strains isolated 

 from human feces. 



Habitat : Probably intestinal canal of 

 mammals. 



13. Bacteroides incommunis Eggerth 

 and Gagnon. (Eggerth and Gagnon, 

 Jour. Bact., 25, 1933, 402; Ristella incom- 

 munis Prdvot, Ann. Inst. Past., 60, 



1938, 291.) From Latin incommunis, 

 not common. 



Rods: 0.5 to 1.5 by 1.0 to 3.0 microns, 

 occurring singly. Stain solidly. Non- 

 motile. Gram-negative. 



Gelatin: Not liquefied. 



Blood agar colonies : Elevated, slightly 

 yellowish, 1 mm in diameter. One 

 strain formed soft colonies; the other 

 was stringy when emulsified. 



Broth: Growth is diffuse. 



Indole not formed. 



Hydrogen sulfide is formed. 



Milk: Acidified but not coagulated; 

 coagulates promptly on boiling. 



Nitrites not produced from nitrates. 



Peptone : No gas . 



Acid and a small amount of gas from 

 amygdalin, arabinose, cellobiose, dextrin, 

 fructose, galactose, glucose, inulin, lac- 

 tose, maltose, mannose, raffinose, rham- 

 nose, sucrose and xylose. One strain 

 fermented glycogen and starch. No 

 action on esculin, glycerol, mannitol, 

 melezitose, salicin, sorbitol or trehalose. 



Non-pathogenic for white mice and 

 rabbits. 



Anaerobic. 



Source : Two strains isolated from hu- 

 man feces. 



Habitat : Probably intestinal canal of 

 mammals. 



14. Bacteroides distasonis Eggerth and 

 Gagnon. (Eggerth and Gagnon, Jour. 

 Bact., 25, 1933, 403; Ristella distasonis 

 Pr^vot, Ann. Inst. Past., 60, 1938, 291.) 

 Named for Distaso, Roumanian bac- 

 teriologist. 



Rods: 0.5 to 0.8 by 1.5 to 2.5 microns, 

 occurring singly. Staining solidly and 

 having rounded ends. Some strains 

 show a few bacilli 5.0 to 8.0 microns long. 

 Non-motile. Gram-negative. 



Gelatin: Not liquefied by 16 strains. 

 The remaining 4 liquefied gelatin in 35 

 to 50 days. 



Blood agar colonies : Soft, grayish, 

 elevated colonies, 1.0 to 1.5 mm in diam- 

 eter. Two strains markedly hemolytic. 



