578 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



22. 1930, 235; Pr^vot, loc. cit., 293.) 

 From river water. For a description of 

 this organism, see IVIanual, 5th ed., 1939, 

 677. 



5. Zuberella variegata (Distaso) Pr^- 

 vot. {Bacillus variegatus Distaso, Cent. 

 f. Bakt., I Abt., Orig., 62, 1912, 445; 

 Bacteroides variegatus Castellani and 

 Chalmers, Man. Trop. Med., 3rd ed., 

 1919, 960; Pr^vot, loc. cit., 293.) From 

 the intestines. For a description of this 

 species, see Manual, 5th ed., 1939, 578. 



6. Zuberella rhinitis (Tunnicliff) Pri- 

 vet. (Bacillus rhinitis Tunnicliff, Jour. 

 Inf. Dis., 16, 1915, 493; Pr^vot, loc. cit., 

 293.) Thirty-two strains isolated from 

 the nasopharynx in human beings suffer- 

 ing from pharyngitis, tonsilitis, bron- 

 chitis and rhinitis, as well as from the 

 nasal mucosa of normal human beings, 

 rabbits, guinea pigs and dogs. For a 

 description of this species, see Manual, 

 5th ed., 1939, 576. 



Family Spherophoraceae Pr^vot. 



(Loc. cit., 289.) 

 Genus I. Spherophorus Prevot. 



{Loc. cit., 297.) 



Very pleomorphic rods. Metachro- 

 matic granules common in elongated 

 forms. Non-motile. Non-spore-form- 

 ing. Gram-negative. 



1. Spherophorus necrophorus (Fliigge) 

 Prevot. (Bacillus der Kalberdiphthe- 

 rie, Loeffler, Mitteil. kaiserl. Gesund- 

 heitsamte, 2, 1884, 493; Bacillus 

 diphtheriae vitulorum Fltigge, Die Milcro- 

 organismen, 2 Aufl., 1886, 265; Bacillus 

 necrophorus Fliigge, ibid., 273; Bacillus 

 diphtheriae-vitulorum Trevisan, I generi 

 e le specie delle Batteriacee, 1889, 13; 

 Bacillus filiformis Shiitz; not Bacillus 

 filiformis Tils, Ztschr. f. Hyg., 9, 1890, 

 294; not Bacillus filiformis Migula, Syst. 



d. Bakt., £, 1900, 587; Nekrosebacillen, 

 Bang, Maanedskrift f . Dyrleager, 2, 1890, 

 235; Streptothrix cuniculi Schmorl, Deut. 

 Ztschr. f. Tiermed., 17, 1891, 376; Acti- 

 nomyces cuniculi Gasperini, Mittheil. 

 11 Internal. Med. Congr. Rome, see 

 Cent. f. Bakt., 15, 1894, 684; not Acti- 

 nomyces cuniculi Erikson, Med. Res. 

 Council Spec. Rept. Ser. 203, 1935, 32; 

 Oospora diphtheriae vitulorum Lehmann 

 and Neumann, Bakt. Diag., 1 Aufl., 2, 

 1896, 393; Actinomyces necrophorus 

 Lehmann and Neumann, Bakt. Diag., 

 2 Aufl., 2, 1899, 434; Streptothrix necro- 

 phora Kitt, Bakterienkunde, 1899; Cory- 

 nebacterium necrophorum Lehmann and 

 Neumann, Bakt. Diag., 4 Aufl., 2, 1907, 

 531 ; Bacillus necroseos Salomonsen, 

 quoted from Lehmann and Neumann, 

 ibid., 532; Cladothrix cuniculi Mac^, 

 Traite de Bact., 6th ed., 2, 1913, 753; 

 Bacterium necrophorum Lehmann and 

 Neumann, Bakt. Diag., 7 Aufl., 2, 1927, 

 504 ; Fusiformis necrophorus Topley and 

 Wilson, Princip. Bact. and Immun., 1st 

 ed., 1, 1931, 299; Prevot, Ann. Inst. Past., 

 60, 1938, 298.) Because of the impor- 

 tance of this organism, a description is 

 included here: 



Rods: 0.5 to 1.5 microns wide, forming 

 long filaments, up to SO to 100 microns 

 long. Some authors report branching, 

 others deny this. Short forms are re- 

 ported by Schmorl to be motile. Gram- 

 negative. 



Gelatin stab : No liquefaction. 



Agar colonies: Small, dirty-white, 

 circular, opaque, with yellowish center 

 under low power lens. Margin floccose. 



Agar stab : Yellowish colonies along 

 needle track. Gas bubbles produced. 



Coagulated blood serum: Small, whit- 

 ish colonies, becoming opaque, fimbriate. 



Broth: Turbid, with gas. Cheese-like 

 odor. 



Indole is formed. 



Litmus milk : Cheese-like odor. Acidi- 

 fied and generally coagulated. 



Nitrites not produced from nitrates. 



Anaerobic. 



