582 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Indole not formed. 



Acid from glucose, fructose, sucrose, 

 maltose and sometimes from lactose. 

 No acid from inulin or mannitol. (Hine 

 and Berry, Jour. Bact., 34, 1937, 524.) 



No H2S produced. 



No odor produced. 



No gas formed. 



Non-pathogenic for white mice (Hau- 

 duroy et al., loc. cit.). 



Temperature relations: Optimum 35° 

 to 37°C. Minimum 30°C. (Hauduroy 

 et al., loc. cit.) 



Optimum pH 6.8 to 8.0 (Hauduroy et 

 al., loc. cit.). 



Anaerobe . 



Source : Two strains isolated from 

 deposit on teeth. 



Habitat : Presumably the buccal cavity. 



2. Fusobacterium biacutum Weinberg 

 and Prdvot. (Weinberg and Prdvot, 

 Compt. rend. Soc. Biol., Paris, 95, 1926, 

 519 ; Fusiformis biacutus Hauduroy et al.. 

 Diet. d. Bact. Path., 1937, 238.) From 

 Latin, double pointed. 



Rods: 0.4 to 0.5 by 1.4 to 3.0 microns, 

 with pointed ends, occurring singly, in 

 pairs or sometimes in short chains. Non- 

 motile. Gram-negative. 



Gelatin : No liquefaction. 



Veillon's agar: Rapid growth. Colo- 

 nies lens-shaped. Gas is produced which 

 breaks up the medium. 



Plain broth: Poor growth. 



Glucose broth: Turbid. Medium 

 rapidly acidified. Good growth. 



Indole not formed. 



Milk: Acid and coagulation in 2 to 8 

 days. Curd not digested. 



Casein and coagulated egg-white not 

 digested. 



Neutral red reduced. 



Acid from glucose, fructose, galactose, 

 maltose and lactose. 



Small amount of H2S produced. 



Does not require blood serum for 

 growth. 



Pathogenic for guinea pigs. 



Killed in 60 minutes at 60°C. 



Anaerobic. 



Source : Six strains isolated from a case 

 of appendicitis. 



Habitat : Unknown. 



3. Fusobacteriiim nucleatum Knorr. 

 (Knorr, Cent. f. Bakt., I Abt., Orig., 

 89, 1923, 17; Fusiformis nucleatus Bergey 

 et al.. Manual, 3rd ed., 1930, 514.) Lat- 

 inized, nucleated. 



Rods: 1.0 by 4.0 microns, spindle- 

 shaped, occurring singly. One or two 

 granules present. Non-motile. Gram- 

 negative. 



Serum agar plate : Deep colonies lens- 

 shaped with offshoots. 



Plain liver broth: No growth. 



Liver broth with serum: After 1 to 3 

 days, flocculent deposit on the pieces 

 of liver. 



Indole not formed (Knorr, loc. cit.). 

 Indole formed (Hine and Berry, Jour. 

 Bact., 34, 1937, 521). • 



Disagreeable odor produced on cul- 

 tivation. 



No gas produced. 



Acid from glucose, usually from fruc- 

 tose, sometimes from sucrose and lactose. 

 No acid from maltose, inulin or mannitol. 

 (Hine and Berry, loc. cit.) 



No H2S formed. 



Temperature relations: Optimum 35° 

 to 37 °C. Minimum 30°C. (Hauduroy 

 et al., Diet. d. Bact. Path., 1937, 239.) 

 Survives 56°C for 15 minutes, but not 

 60°C for 10 minutes (Hine and Berry, 

 loc. cit.). 



Optimum pH 6.8 to 8.2 (Hauduroy et 

 al., loc. cit.). 



Anaerobe. 



Source : One strain isolated from de- 

 posit on teeth in a healthy mouth. 



Habitat : Presumably the buccal cavity. 



4. Fusobacterium polymorphum Knorr. 

 (Knorr, Cent. f. Bakt., I Abt., Orig., 89, 

 1923, 19; Fusiformis polymorphus Bergey 

 et al.. Manual, 3rd ed., 1930, 515.) From 

 Greek, assuming many forms. 



Rods: 0.2 to 0.5 by 8 to 16 microns. 



