592 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Biol, and Med., 3, 1945, 187.) From 

 Latin hovis, of the ox. 



Short, plump rods: 0.5 by 1.5 to 2.0 

 microns, usually occurring in pairs and 

 short chains, with rounded ends. Cap- 

 sulated. Non-motile. Gram-negative. 



Gelatin: Slow growth at 22°C. Very 

 slow liquefaction. 



Blood agar colonies : After 24 hours, 

 round, translucent, grayish-white, sur- 

 rounded by a narrow, clear zone of hem- 

 olysis. Deep colonies tiny with a clear 

 hemolytic zone, usually 1.5 mm in diam- 

 eter. After 48 hours, surface colonies 

 somewhat flattened, 3.5 to 4 mm in 

 diameter; deep colonies ellipsoidal and 

 biconvex with hemolytic area of 2.5 to 

 3 mm in diameter. 



Blood agar slants: After 24 hours at 

 38°C, heavy, viscid, grayish-white 

 growth . 



Coagulated serum liquefied. 



Broth: Slow growth. Slight turbidity. 

 Considerable sediment. 



Litmus milk: Alkaline. Partial co- 

 agulation. 



Indole not produced. 



Potato: No growth. 



No acid from glucose or other carbo- 

 hydrates. 



Not pathogenic for laboratory animals. 



Killed at 58° to 59°C in five minutes. 



Aerobic. 



Source : From cases of acute ophthalmia 

 (pink eye) of cattle. 



Habitat : In the exudate from cases of 

 acute ophthalmia of cattle. The prob- 

 able cause of bovine infectious keratitis 

 (Baldwin, Amer. Jour. Vet. Res., 6, 

 1945, 180). 



Appendix: Other species placed in the 

 genus Moraxella are as follows : 



Moraxella josephi Lwoff. (Bacillus 

 duplex josephi Scarlett, Annales d'Ocu- 

 listique, 153, 1916, 100 and 485; Lwoff, 

 Ann. Inst. Past., 62, 1939, 171; Moraxella 

 duplex josephi Lwoff, ibid., 174; Bacillus 

 josephi Audureau, Ann. Inst. Past., 64, 

 1940,126.) Gram-positive. Pathogenic. 

 From the conjunctiva of man. 



Moraxella Iwoffi Audureau. (Ann. 

 Inst. Past., 64, 1940, 150.) Two varie- 

 ties are recognized : var. bacteroides and 

 var. brevis. From various types of 

 conjunctivitis in man. 



Moraxella non liquefaciens Lwoff. 

 (Bacterium duplex-nonliquefaciens Oli- 

 ver and Wherry, Jour. Inf. Dis., 28, 

 1921, 342; Bacillus duplex non-lique- 

 jaciens Hewlett, Med. Res. Council 

 Syst. of Bact., 2, 1929, 418; Lwoff, Ann. 

 Inst. Past., 62, 1939, 171; Moraxella 

 duplex non liquefaciens Lwoff, ibid., 

 174; Bacilhis duplex non liquefaciens 

 Audureau, Ann. Inst. Past., 64, 1940, 

 126; Moraxella duplex var. non liquefa- 

 ciens Audureau, ibid., 144.) From an 

 ulcer of the cornea, and from bronchial 

 sputum in man. 



Genus III. Noguchia Olitsky, Syverton and Tyler.* 



(Jour. Exp. Med., 60, 1934, 382.) Named for Noguchi, the bacteriologist who iso- 

 lated the type species. 



Small, slender. Gram-negative rods present in the conjunctiva of man and animals 

 affected by a follicular type of disease ; mucoid type of growth which on first isolation 

 takes place with some difficulty in ordinary media; motile, flagellated, and encap- 

 sulated; aerobic and facultative anaerobic; optimum temperature for growth 28° to 

 30°C. 



The type species is Noguchia granulosis (Noguchi) Olitsky, Syverton and Tyler. 



Key to the species of genus Noguchia. 



I. Acid from carbohydrates. 



A. Acid from raffinose, maltose and salicin. 



1. Noguchia granulosis. 



Arranged by Prof. CD. Kelly, McGill University, Montreal, October, 1938. 



