FAMILY PARVOBACTERIACEAE 



593 



B. No acid from raffinose, maltose and salicin. 



2. Noguchia simiae. 

 II. Xo acid from carbohydrates. 



3. Noguchia cuniculi. 



1. Noguchia granulosis (Xoguchi) 

 Olitsky et al. {Bacterium granulosis 

 Xoguchi, Jour. Exp. Med., J^8, Supp. 2, 

 1928, 21; Olitsky, Syverton and Tyler, 

 Jour. Exp. Med., 60, 1934, 382.) From 

 Latin, granular. 



Rods : 0.25 to 0.3 by 0.8 to 1.2 microns, 

 motile by means of a single flagellum, 

 usually polar. Pleomorphic. Gram-neg- 

 ative. 



Xo growth on plain agar or broth. 

 Blood agar plate : Minute round col- 

 onies, shiny, somewhat raised, almost 

 transparent or slightly grayish in 48 

 hours. Later the colonies increase in 

 size, are grayish opalescent and some- 

 what sticky. Old colonies have a brown- 

 ish or yellowish tint. 



Semi-solid Leptospira medium : Gray- 

 ish-white, diffuse growth, forming a 

 delicate zone 1 cm deep. 



Liquid Leptospira medium: Diffuse, 

 slightl}' cloudy growth, with sticky 

 grayish sediment at the bottom of the 

 tube in old cultures. 



Acid from glucose, fructose, niannose, 

 sucrose, galactose, maltose, salicin, 

 xylose, mannitol, de.xtrin, arabinose, 

 amj'gdalin and lactose. Small amount 

 of acid from raffinose, inulin, rhamnose 

 and trehalose. Xo acid from dulcitol, 

 sorbitol and inositol . 



Non-pathogenic for rabbits, guinea 

 pigs, rats and mice. 

 Optimum pH 7.8. 



Temperature relations: Optimum 15" 

 to 30°C. Grows at 37°C. 

 Aerobe, facultative anaerobe. 

 Distinctive characters : Action on car- 

 bohydrates; agglutination reactions; mo- 

 tility at 15°, none at 37 =C. 



Source : From trachoma of American 



Indians at Albuquerque, Xew Mexico. 



Habitat : Regarded by Xoguchi and 



others as a cause of trachoma in man. 



Produces a granular conjunctivitis in 

 monkej's and apes. 



2. Noguchia simiae Olitsky et al. 

 (Bacterium simiae Olitsky, Syverton 

 and Tyler, Jour. Exp. Med., 57, 1933, 

 875; Olitsky et al.. Jour. Exp. Med., 

 60, 1934, 382.) From Latin simia, ape. 



Slender rods: 0.2 to 0.3 by 0.8 to 1.2 

 microns, occurring singly, in pairs, in 

 short chains or parallel arrangement of 

 two or three, having pointed ends. Cap- 

 sules are found. Actively motile by 

 means of a single, rarely a double, flagel- 

 lum, usually polar. Gram-negative. 



Gelatin plates : Colonies more mucoid 

 and raised than on agar. 



Gelatin stab : Arachnoid growth along 

 line of inoculation. Xo liquefaction. 



Agar plates: Small, circular, grayish, 

 translucent, smooth, convex, slightly 

 raised colonies having a sticky or mucoid 

 consistencj'. 



Blood agar plates : More highly translu- 

 cent and colorless in early growth than 

 on plain agar, becoming grayish after two 

 or three daj's. 



Agar slants: Grayish-white to white, 

 moist, mucoid, raised, glistening growth. 

 Growth is more profuse when blood is 

 added. 



Leptospira medium : Homogeneous, 

 dense growth in a 0.5 cm sharpl}^ defined 

 layer, -nith a slight, nebulous, uniform 

 opacity about 1 cm below. In three or 

 four days the lower layer becomes more 

 dense and in time extends to the bottom 

 of the tube. 



Broth : Uniform turbidit}', with a slight 

 grayish sediment and no pellicle. 



Litmus milk: Unchanged. 



Potato : Light tan, spreading, abundant 

 growth. 



Indole not formed. 



Xitrites not produced from nitrates. 



Acid but no gas from glucose, fructose. 



