594 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



mannose, galactose, xylose, arabinose 

 and rhamnose. Small amount of acid 

 from dextrin. Some strains produce a 

 small amount of acid from sucrose, lac- 

 tose, inulin and mannitol. Raffinose, 

 salicin, dulcitol, amygdalin, maltose, 

 trehalose, sorbitol and inositol un- 

 changed. 



Serological reactions: Rabbit anti- 

 serum is specific for all strains and no 

 cross agglutination with Noguchia gran- 

 ulosis. 



Temperature relations : Optimum 28° 

 to 30°C. Thermal death point 56°C for 

 thirty minutes. 



Aerobe, facultative anaerobe. 



Distinctive characters : Action on car- 

 bohydrates; agglutination reactions. 



Source : From inflammatory type (Type 

 II) of spontaneous conjunctival follicu- 

 losis in Macacus rhesus monkeys. 



Habitat : Causes conjunctival follicu- 

 losis in Macacus rhesus monkeys. 



3. Noguchia cuniculi Olitsky, Syver- 

 ton and Tyler. (Jour. Exp. Med., 60, 

 1934, 382. ) From Latin cuniculus, rabbit . 



Slender rods: 0.2 to 0.3 by 0.5 to 1.0 

 micron with pointed ends. Capsules 

 are formed of much finer texture than 

 those surrounding Noguchia granulosis 

 or Noguchia simiae. Actively motile 

 with peritrichous flagella. Non-acid- 

 fast. Pleomorphic forms sometimes 

 noted. Gram-negative. 



Gelatin agar plates: Grayish, mucoid 

 and confluent colonies. 



Gelatin stab: Tenuous, arborescent, 

 non-spreading growth. No liquefaction. 



Agar plates: Small, spherical, translu- 

 cent, slightly grayish, smooth, some- 

 what convex, moist and mucoid colonies 

 with entire edges. 



Blood agar plates: More profuse, more 

 grayish and less translucent than on plain 

 agar. 



Agar slants: Slightly grayish, translu- 

 cent, coalescent, glistening, mucoid, 

 homogeneous and non-spreading growth. 

 The water of syneresis appears uniformly 

 cloudy or milkj^ depending on amount of 

 growth. 



Leptospira medium: After 24 hours, a 

 faint, nebulous surface growth followed 

 by an ingrowing sac -like mass, with its 

 base 5 mm across, lying at the center of 

 the under surface and extending for 5 

 mm into the medium. The area spreads 

 laterallj^ until at about two or three days 

 there is a uniform, opaque, whitish 

 layer about 1 cm thick which progresses 

 slowly until the bottom of the tube is 

 reached in about seven days. 



Broth : Uniform turbidity, without 

 pellicle. 



Litmus milk: Unchanged. 



Potato : Faint, buff-colored (changing 

 to brown after five days), non-spreading, 

 sparse surface growth. 



Indole not produced. 



Nitrites not produced from nitrates. 



No acid or gas from glucose, fructose, 

 inannose, mannitol, sucrose, raffinose, 

 inulin, galactose, maltose, salicin, xylose, 

 dextrin, arabinose, amygdalin, lactose, 

 dulcitol, rhamnose, trehalose, sorbitol or 

 inositol. 



Serological relations : Rabbit antiserum 

 is specific for all strains, and no cross 

 agglutination with Noguchia granulosis 

 or Noguchia simiae. 



Temperature relations : Optimum 28° 

 to 30°C. Thermal death point 56°C for 

 15 to 30 minutes. 



Aerobe, facultative anaerobe. 



Distinctive characters : No action on 

 carbohydrates; peritrichous flagella; ag- 

 glutination. 



Source : From spontaneous conjunctival 

 folliculosis, Type II of rabbits. 



Habitat : Causes conjunctival follicu- 

 losis in rabbits. 



Genus IV . Dialister Bergey el al.* 

 (Manual, 1st ed., 1923, 271.) 

 Minute rod-shaped cells, occurring singly, in pairs and short chains. Non-motile. 



Rearranged by Prof. D. H. Bergey, Philadelphia, Pennsylvania, 1933. 



