604 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Litmus milk: Coagulated, becoming 

 alkaline. 



Potato: Dirty -yellowish, limited 

 streak. 



Indole not formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 20°C. 



This species is selected as the type 

 species for the genus Zcitnowia Ender- 

 lein {loc. cit.). 



Source: Contamination on agar plate. 



Habitat: Unknown. 



8. Bacterium healii Buchanan and 

 Hammer. (Buchanan and Hammer, 

 Iowa Agr. Exp. St a. Research Bull. 22, 

 1915, 249; Escherichia healii Bergey 

 et al., Manual, 1st ed., 1923, 200; Achru- 

 mohacter healii Bergey et al., Manual, 

 2nd ed., 1925, 157.) 



Rods: 0.5 to 0.7 by 2.2 to 12.9 microns, 

 occurring singly and in short chains. 

 Non-motile. Gram-positive. 



Gelatin stab: Stratiform litiuefaction. 

 Villous growth in stab. 



Agar colonies: Large, white, rhizoid. 



Agar slant: White, hard. growth, with 

 no tendencj' to stringiness. 



Broth: Gray pellicle and sediment. 



Litmus milk: Slightly acid, becoming 

 slimy, coagulated, peptonized. 



Potato: Heavy, white, glistening 

 growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Acid without gas from glucose, fruc- 

 tose, maltose, sucro.se, salicin and starch. 

 No acid from mannitol, lactose, raffinose 

 or inulin. 



Aerobic, facultative. 



Optimum temperature 22°C. 



Source: Slimy milk. 



Habitat : Unknown. 



9. Bacterium insectiphilium Steinhaus. 

 (Jour. Bavi.. 42, 1941, 777.) From M. 

 L. insect, insect and Greek philos, 

 loving. 



Rods: 0.8 to 1.2 by 1.0 to 2.8 microns, 

 occurring singly. At times appearing 



almost as cocci or coccobacilli. Non- 

 motile. Gram -positive. 



Gelatin stab: Liquefaction. 



Agar colonies: Light greenish-yellow, 

 circular, entire, raised, glistening, 

 smooth, opaque. 



Agar slant: Filiform, raised, smooth, 

 glistening, opaque growth. 



Broth: Moderate turbidity, slight vis- 

 cid sediment. 



Litmus milk: Alkaline, peptonization, 

 and slow reduction. 



Potato: Greenish-yellow, thick, moist 

 growth . 



Indole not produced. 



Nitrites not produced from nitrates. 



Hydrogen sulfide not produced. 



Starch slightly hj-drolj'zed. 



No action on the following carbohy- 

 drates: Glucose, lactose, sucrose, mal- 

 tose, fructose, mannitol, galactose, arab- 

 inose, xylose, dextrin, salicin, rhamnose, 

 raffinose, trehalose, sorbitol, inulin, dul- 

 citol, glycerol, adonitol, mannose. 



Aerobic. 



Source: From the body wall of the 

 bagworm, Thyridopteryx ephemeraefor- 

 juis Haw. 



Habitat : Unknown. 



10. Bacterium tegumenticola Stein- 

 haus. (Jour. Bact., ^2, 1941, 775.) 

 From Latin legumenlum, cover, skin 

 and cola, dweller. 



Small rods: 0.5 to 0.8 by 1.0 to 1.5 mi- 

 crons. Have a tendency to be ellipsoidal 

 on solid media. Non-motile. Gram- 

 positive. 



Gelatin stab: Generally no liciuefac- 

 tion. ^'ariable. 



Agar colonies: Tiny (1 nmi), white, 

 convex, glistening, circular, entire. 



Agar slant: Filiform, glistening, gray- 

 ish-white growth. 



Broth: Slight turbidity; sediment. 



Litmus milk: No change. 



Indole not produced. 



Hydrogen sulfide not produced. 



Nitrites not produced from nitrates. 



Starch not hydrolyzed. 



Acid slowly produced from glucose 



