606 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



nitol. No acid from maltose, lactose, 

 sucrose, glycerol, xylose or salicin. 



Starch is hydrolyzed. 



Hydrogen sulfide not formed. 



Ammonia produced from peptone but 

 not from urea. 



Casein is digested. 



Fats are not hydrolyzed. 



Aerobic, facultative. 



Optimum temperature 20° to 25°C. 



Source: Found on the skin of marine 

 fish. 



Habitat: Not known from other 

 sources. 



14. Bacterium sociovivum ZoBell and 

 Upham. (Bull. Scripps Inst. Ocean- 

 ography, La Jolla, 5, 1944, 269.) From 

 Latin socius, associate and vivum, to live. 



Rods: 0.5 to 0.8 by 3.0 to 4.0 microns, 

 with rounded ends, occurring singly, in 

 pairs, and chains. Non-motile. Gram- 

 positive but tends to destain, leaving 

 Gram-positive cell wall and granules. 



All differential media except the fresh- 

 water broth, litmus milk and potato were 

 prepared with sea water. 



Gelatin colonies: Irregular, sunken, 

 filamentous margin, grayish-white. 



Gelatin stab: Crateriform liquefaction 

 becoming stratiform. 



Agar colonies: 2 to 4 mm, circular, 

 convex, smooth, entire, darker center. 



Agar slant: Luxuriant, beaded, glis- 

 tening, butj'rous growth with no pig- 

 ment. 



Sea-water broth: No pellicle, no tur- 

 bidity, heavy flocculent sediment. 



Fresh -water broth: Fair growth. 



Litmus milk: Decolorized, neutral, 

 completely peptonized in 20 days. 



Potato: Abundant, dull, light cream- 

 colored growth. Potato darkened. 



Indole not formed. 



Nitrites not produced from nitrates. 



Acid but no gas from glucose, maltose, 

 and mannitol. No acid from glycerol, 

 lactose, sucrose, or salicin. 



Starch is hydrolyzed. 



Hydrogen sulfide not formed. 



Ammonia produced from peptone but 

 not from urea. 



Casein is digested. 



Fats not hydrolyzed. 



Aerobic, facultative. 



Optimum temperature 20° to 25°C. 



Source: Found associated with seden- 

 tary organisms in the sea. 



Habitat: Commonly found on sub- 

 merged surfaces and on sessile diatoms 

 in sea water. 



15. Bacteritim immotum ZoBell and' 

 Upham. (Bull. Scripps Inst. Ocean- 

 ography, 5, 1944, 271.) From Latin, 

 meaning immobile or stationary. 



Rods: 0.8 by 3.1 to 8.6 microns, with 

 rounded ends, occurring singly, in pairs, 

 and long chains. Non -motile. Gram- 

 positive but tend to destain leaving 

 Gram-positive outline and granules. 



All differential media except the fresh- 

 water broth, litmus milk, and potato 

 were prepared with sea water. 



Gelatin colonies:^ Small, circular, 

 raised, gray, slowly digest gelatin. 



Gelatin stab: Crateriform liquefaction 

 becoming infundibuliform. Beaded 

 growth along line of stab. No pigment. 



Agar colonies : 1 to 2 mm, circular, con- 

 vex, smooth, lobate margin, darker 

 centers. 



Agar slant: Luxuriant, glistening, 

 echinulate, mucoid growth with no 

 pigment. 



Sea-water broth : No pellicle, moderate 

 turbidit}', abundant, flocculent sedi- 

 ment. 



Fresh-water broth: Scanty growth. 



Litmus milk: Decolorized, neutral, 

 partly peptonized in 20 days. 



Potato: Luxuriant, mucoid, creamy 

 growth which darkens potato. 



Indole not formed. 



Nitrites not produced from nitrates. 



Acid but no gas from glucose, maltose, 

 xylose, and mannitol. No acid from 

 glycerol, lactose, sucrose, or salicin. 



Starch is hydrol3^zed. 



Hvdrogen sulfide not formed. 



