FAMILY BACTERIACEAE 



607 



Ammonia produced from peptone but 

 not from urea. 



Casein is digested. 



Fats not hydrolyzed. 



Aerobic, facultative. 



Optimum temperature 20° to 25°C. 



Source: Found associated with marine 

 sedentary organisms. 



Habitat: Xot known from other 

 sources. 



16. Bacterium ammoniagenes Cooke 

 and Keith. (Cooke and Keith, Jour. 

 Bact., 13, 1927, 315; Alcaligenes ammoni- 

 agenes Bergey et al., Manual, 3rd ed., 

 1930, 367.) From M. L. ammonia and 

 Latin genera, develop. 



Rods with rounded ends, 0.8 by 1.4 

 to 1.7 microns, occurring singly. Xon- 

 motile. Gram-positive. 



Gelatin stab: Xo liquefaction. 



Agar colonies: Circular, flat, smooth, 

 entire, gray. 



Agar slant : Growth moderate, smooth, 

 flat, opaque, glistening, butyrous, amor- 

 phous. 



Broth: Moderate turbidity, with floc- 

 culent sediment. 



Litmus milk: Slightly alkaline. 



Indole not formed. 



Xo action on carbohydrates. 



Blood serum not liquefied. 



Urea is fermented forming ammcmia. 



Aerobic, facultative. 



Optimum temperature 30°C. 



Source: From feces of infants. 



Habitat: Presumably widely dis- 

 tributed in putrefying materials. 



17. Bacteriimi minutissimum Migula. 

 (Bacillus pyogenes miriutissimus Kruse, 

 in Fliigge, Die Mikroorganismen, 3 Aufl., 

 2, 1896, 447; Bacterium pyogenes minu- 

 tissimus Chester, Ann. Rept. Del. Col. 

 Agr. Exp. Sta., 9, 1897, 89; Migula, 

 Syst. d. Bakt., 2, 1900, 418; Eberlhella 

 minutissima Bergey et al.. Manual, 1st 

 ed., 1923, 228; Shigella minutissima 

 Bergey et al., Alanual, 3rd ed., 1930, 

 359.) From Latin, smallest. 



Description from Kruse {loc. cit.). 

 Description not different from that of 

 Bacillus tenuis sputigenes Pansini, ac- 

 cording to Chester (Ann. Rept. Del. 

 Col. Agr. Exp. Sta., 9, 1897, 89). 



Rods: 0.5 by 1.0 micron, occurring 

 singly and in pairs. Xon-motile. 

 Gram-positive. 



Gelatin stab: Xo liquefaction. Yel- 

 lowish growth spreading slightly on 

 surface. 



Growth on agar and blood serum is not 

 characteristic. 



Acid but no gas from glucose and lac- 

 tose. 



Xo characteristic odor. 



Xot pathogenic for mice and rabbits. 



Aerobic, facultative. 



Optimum temperature 37 °C. 



Source: Isolated from a facial abscess. 



Habitat: Xot known from other 

 sources. 



18. Bacteriiun incertum Steinhaus. 

 (Jour. Bact., 42, 1941, 776.) From Latin 

 incertus, uncertain. 



Short rods: 0.5 to 0.8 by 1.0 to 1.5 

 microns, occurring singly and occasion- 

 ally in pairs. Young cultures motile, 

 after 48 hours generally non-motile. 

 Gram-positive; after 48 hours many 

 cells become Gram-negative. 



Gelatin stab: Xo liquefaction. 



Agar colonies: Tiny, grayish-white, 

 smooth, almost transparent. Does not 

 grow well on nutrient agar. 



Xorth's gelatin chocolate agar slant: 

 Filiform, thin, transparent growth. 

 Brown color of chocolate medium 

 changes to yellowish-green. 



Blood agar: Alpha hemolysis at first; 

 after three days beta hemolysis. 



Broth: Almost clear; ver\- slight 

 growth. 



Litmus milk: Xo change. 



Indole not produced. 



Hydrogen sulfide not produced. 



Xitrites not produced from nitrates. 



Starch not hj'drolyzed. 



Acid but no gas from glucose, sucrose. 



