608 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



fructose, inannose, and maltose. No 

 fermentation of lactose, rhamnose, galac- 

 tose, mannitol, dulcitol, inositol, or 

 sorbitol. 



Voges-Proskauer test: Negative. 



Microaerophilic. 



Source: From the ovaries of the lyre- 

 man cicada, Tibicen linnei Smith and 

 Grossbeck. 



Habitat: Unknown. 



19. Bacterium imperiale Steinhaus. 

 (Jour. Bact., ^2, 1941, 777.) From Latin 

 imperialis, referring to the imperial 

 moth. 



Small rods: 0.5 to 0.8 by 1.0 to 1.7 

 microns, occurring singly and in pairs. 

 A few cells motile in young cultures. 

 Gram-positive. 



Gelatin stab: No liquefaction. 



Agar colonies: Circular, entire, almost 

 translucent, pinkish-orange to yellow 

 pigment. 



Agar slant: Filiform, glistening, 

 opaque growth. 



Broth: Slight to moderate turbidity; 

 slight sediment. 



Litmus milk: No change at first, later 

 slightly acid. 



Potato: Heavy, glistening, moist 

 growth; reddish to yellowish-orange. 



Indole not produced. 



Hydrogen sulfide not produced. 



Nitrites not produced from nitrates. 



Starch not hydrolyzed. 



Acid but no gas from glucose, sucrose, 

 maltose, fructose, mannitol, galactose, 

 arabinose, xylose, salicin, raffinose, tre- 

 halose, sorbitol, mannose, adonitol, 

 esculin, and slight acid from lactose and 

 dextrin. Inuliii, dulcitol, glycerol, 

 rhamnose, adonitol, and inositol not 

 fermented. 



Aerobic. 



Source: From the alimentary tract of 

 the imperial moth, Eacles ■imperialis 

 Dru. 



Habitat: Unknown. 



20. Bacterixmi zopfii Kurth. (Kurth, 

 Bericht. d. deutsch. Botan. Gesellschaft, 



/, 1883, 97; Kurthia zopfii Trevisan, Atti 

 della Accad. Fisio-Medico-Statistica in 

 Milano, Ser. 4, 8, 1885, 92; Helikobac- 

 terium zopfii Escherich, Miinch. med. 

 Wchnschr., 33, 1886, 2, quoted from En- 

 lows, U. S. Hygienic Lab. Bull. 121, 1920, 

 47; Bacterium (Proteus) zopfii Chester, 

 Ann. Rept. Del. Col. Agr. Exp. Sta., 9, 

 1897, 103; Bacillus zopfii Migula, Syst. 

 d. Bakt., 2, 1900, 815; Zopfius zopfii 

 Wenner and Rettger, Jour. Bact., 4, 1919, 

 334.) Named for W. Zopf, German 

 botanist. 



This is the type species of the genus 

 Kurthia Trevisan. (Trevisan, too. cit.; 

 Zopfius Wenner and Rettger, Jour. 

 Bact., 4, 1919, 334.) 



Rods : 0.8 by 3.5 microns, with rounded 

 ends, occurring in long curved chains. 

 Motile with peritrichous flagella. 

 Gram-positive. 



Gelatin colonies: Radiate, filamentous, 

 gray. 



Gelatin stab: Arborescent growth in 

 stab. No liquefaction. 



Agar colonies: Fimbriate. 



Agar slant: Spreading, gray, fimbriate 

 growth . 



Broth: Slow, moderate growth. 



Litmus milk: No change. 



Potato: Moderate, gray growth; me- 

 dium becoming dark. 



No HoS produced. 



Indole not formed. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 25° to 30°C. 



Habitat: Decomposing materials. 



21. Bacterium zenkeri (Hauser) Ches- 

 ter. (Proteus zenkeri Hauser, Ueber 

 Faulnissbakterien, 1885; Bacillus zenkeri 

 Trevisan, I generi e le specie delle Bat- 

 teriacee, 1889, 17; Chester, Ann. Rept. 

 Del. Col. Agr. Exp. Sta., 9, 1897, 103; 

 Zopfius zenkeri Wenner and Rettger, 

 Jour. Bact., 4, 1919, 334; Bacillus pro- 

 teus-zenkeri Holland, Jour. Bact., 5, 

 1920, 220; Kurthia zenkeri Bergey et al., 

 Manual, 2nd ed., 1925, 215.) Named 

 for K. Zenker, German pathologist. 



