FAMILY BACTERIACEAE 



609 



Rods: 0.65 by 1.6 to 2.3 microns, occur- 

 ring in pairs and in chains. Motile 

 with peritrichous flagella. Gram-posi- 

 tive. 



Gelatin colonies: Feather}^, with fila- 

 ments extending in all directions. 



Gelatin stab: Surface growth like 

 colonies. Xo arborescent growth in 

 stab. Xo liquefaction. 



Agar colonies: Thin, filamentous, 

 spreading, grayish. 



Agar slant: Thin, bluish-gray, fila- 

 mentous growth. 



Broth: Slightly turbid, with gray 

 sediment. 



Litmus milk: Xo change. 



Potato: Barely visible, yellowish- 

 gray, glistening growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Xo H2S formed. 



Aerobic, facultative. 



Optimum temperature 30°C. 



Habitat: Decomposing materials. 



Note: Wenner and Rettger, loc. cit., 

 consider the last two species to be 

 identical. 



Appendix I: The following Gram-posi- 

 tive, motile species may belong with the 

 above group. All have been placed at 

 one time or another in the genus Achro- 

 mobactcr or in the genus Flavohacterium. 



1. Achromobacter lipolyticum (Hussi 

 Bergey et al. {Bactridium lipolyticum 

 Huss, Cent. f. Bakt., II Abt., 20, 1908, 

 474; Bergey et al.. Manual, 2nd ed., 

 1925, 158.) From Greek, fat -dissolving. 



Small, oval rods: 0.3 to 0.5 by 0.7 to 

 1.4 microns. Motile, possessing peri- 

 trichous flagella. Gram -positive. 



Gelatin colonies: Circular, grayish to 

 transparent with irregular margin. 



Gelatin stab: Infundibuliform lique- 

 faction. 



Agar colonies: Growth circular, gray, 

 smooth, glistening, with entire margin. 



Broth : Turbid with granular sediment. 



Litmus milk: Coagulated, peptonized, 

 becoming alkaline. 



Potato: Moist, glistening, grajash 

 growth. 



Indole is formed. 



Acid from glucose, sucrose, raflSnose, 

 xylose, mannitol and glycerol. 



Fats are split in milk, giving rise to a 

 rancid odor and a bitter taste. 



Aerobic, facultative. 



Optimum temperature 35°C. 



Source : From the udder of a cow giving 

 abnormal milk. 



Habitat: Milk. 



2. Achromobacter stearophilum (Wein- 

 zirl) Bergey et al. (Bacillus stearo- 

 philus Weinzirl, Jour. Med. Res., S9, 

 1919, 404; Bergey et al., Manual, 1st 

 ed., 1923, 145.) 



Rods: 0.8 by 5.0 microns, occurring 

 singly. Motile. Gram-positive. 



Gelatin colonies: Scanty development. 



Pumpkin gelatin stab : Filiform growth 

 in stab. X'o liquefaction. 



Pumpkin agar colonies : Small, smooth, 

 convex, gra}', entire. 



Pumpkin juice: Slightly turbid. 



Pumpkin milk: Acid, coagulated. 



Potato: Slight, smooth, gray, glisten- 

 ing, fUiform growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



No acid from carbohydrate media. 



Starch from pumpkin hydrolyzed. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Canned pumpkin. 



Habitat: L'nknown. 



3. Achromobacter sulfureum Bergey et 



al. (Bakt. 4, Rubentschick, Cent. f. 

 Bakt., II Abt., 72, 1927, 123; Bergey 

 et al., Manual. 3rd ed., 1930, 220.) 



Rods: 0.7 to 0.8 by 1.7 to 2.2 microns, 

 occurring singlj' and in pairs. Motile. 

 Gram-positive. 



Gelatin stab: Saccate liquefaction. 



Agar colonies : Circular, grayish -white, 

 flat, homogeneous. 



Agar slant: Filiform, grayish -white, 

 smooth, homogeneous growth. Metallic 

 luster. 



