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MANUAL OF DETERMINATIVE BACTERIOLOGY 



Broth: Turbid. 



Litmus milk: Peptonized. 



Potato: Yellowish -brown layer. 



Indole not formed. 



Nitrites produced from nitrates with 

 gas formation. 



Hydrogen sulfide formed. 



Ammonia formed. 



Urea is attacked. 



Methylene blue reduced. 



Aerobic, facultative. 



Optimum temperature 30° to 33°C. 

 Can grow at 0°C. 



Source: Sewage filter beds. 



Habitat: Putrefying materials. 



Note : See Pseudomonas urease Bergey 

 et al. for another motile, Gram-positive 

 organism described by Rubentschick 

 (Bakt. 3) from the same source. 



4. Achromobacter aerophilum (Rubent- 

 schick) Bergey et al. (Urobacterium 

 aerophilum Rubentschick, Cent, f . Bakt., 

 II Abt., 64, 1925, 168; Bergey et al., 

 Manual, 3rd ed., 1930, 224.) 



Rods : 0.75 to 0.85 by 2.0 to 4.5 microns, 

 occurring singly, in pairs, and in chains. 

 Motile. Gram-positive. 



Urea gelatin colonies: Small, circular, 

 dirty -gray, entire. 



Urea gelatin stab: No liquefaction. 



Urea agar colonies: Circular, grayish, 

 smooth. 



Urea agar slant : Dirty-gray, glistening 

 to dry growth. 



Urea broth: Turbid. 



Urea milk: Unchanged. 



Urea potato: Slight, grayish-white 

 streak. 



Indole not formed. 



Nitrites produced from nitrates. 



H2S not formed. 



Ammonia not formed. 



Aerobic, facultative. 



Source: Sewage slime. 



Habitat: Putrefying materials. 



5. Achromobacter citrophilum (Ru- 

 bentschick) Bergey et al. (Urobacterium 

 citrophilumRubentschiek, Cent, f . Bakt., 

 II Abt., 64, 1925, 168; 66, 1926, 161; 67, 



1926, 167; 68, 1926, 327; Bergey et al., 

 Manual, 3rd ed., 1930, 224.) 



Rods : 0.75 to 0.85 by 2.5 to 6.0 microns, 

 occurring singly and in pairs. Motile. 

 Gram-positive. 



Urea gelatin colonies: Small, grayish- 

 white, smooth, undulate. 



Urea gelatin stab: No liquefaction. 



Urea agar slant: Filiform, grayish- 

 white, thin, dry growth. 



Urea broth: Turbid. 



Urea milk: Unchanged. 



Urea potato: Dirty-gray, thin streak. 



Indole not formed. 



Nitrites produced from nitrates. 



Hydrogen sulfide not formed. 



Ammonia not formed. 



Can derive oxygen from sodium ci- 

 trate. 



Aerobic, facultative. 



Optimum temperature 30°C. 



Source: Sewage slime. 



Habitat: Putrefying materials. 



6. Flavobacterium sulfureum Bergey 



et al. {Bacterium punctans suljureum 

 Zettnow, Cent. f. Bakt., I Abt., Orig., 

 77, 1916, 222; Bergey et al., Manual, 1st 

 ed., 1923, 103.) 



Rods: 0.5 to 0.7 by 0.7 to 1.5 microns. 

 Motile, possessing peritrichous flagella. 

 Gram-positive. 



Gelatin colonies: Very small, barely 

 visible, becoming brownish -yellow, 

 granular. 



Gelatin stab: Spreading growth on the 

 surface only. Later crateriform lique- 

 faction. 



Agar slant: Sulfur-yellow growth. 



Broth: Turbid. 



Litmus milk: Alkaline, peptonized, 

 yellow. 



Potato: Sulfur-yellow streak. 



Indole not formed. 



Nitrites not produced from nitrates. 



Blood serum: Sulfur-yellow growth. 

 Partial liquefaction. 



Xo acid from glucose. 



Aerobic, facultative. 



Optimum temperature 25°C. 



