614 



MANUAL OF DETERMIXATIVE BACTERIOLOGY 



Gelatin stab: Moderate, yellowish 

 growth. Slight napiform liquefaction. 



Agar colonies: Circular, convex, soft, 

 becoming brittle, grayish, granular, en- 

 tire. 



Agar slant: Scant, yellowish-white 

 growth, becoming distinctly yellow. 



Ammonia cellulose agar: Enzymatic 

 zone shows a diameter of 2 to 3 mm at 

 the end of 30 days. 



Peptone cellulose agar: Enzymatic 

 zone shows a diameter of 1.5 to 2.0 mm 

 at the end of 30 days. 



Broth: Turbid. 



Filter paper broth : Paper reduced to 

 thin, limp sheet which falls apart on 

 slight agitation at end of 15 days. 



Litmus milk: Acid, not digested. 



Potato: Abundant, moist, glistening, 

 grayish -white growth, becoming dis- 

 tinctly yellow. 



Indole not formed. 



Nitrites produced from nitrates. 



Ammonia not produced. 



Acid from glucose, maltose, lactose, 

 starch and glycerol. 



Aerobic, facultative. 



Optimum temperature 2G°C. 



Source: Soil from California. 



Habitat: Soil. 



23. Bacterium udum Kellerman et al. 

 (Kellerman, McBeth, Scales and Smith, 

 Cent. f. Bakt., II Abt., 39, 1913, 514; 

 Cellulomonas uda Bergey et al.. Manual, 

 1st ed., 1923, 166; Proteus cellulomonas 

 var. Proteus udus Pribram, Klassifika- 

 tion der Schizomyceten, Leipzig und 

 Wien, 1933, 72.) From Latin mlus grow- 

 ing in marshy ground. 



Rods: 0.5 by 1.5 microns. Non -mo- 

 tile. Gram-negative. 



Gelatin stab: Liquefaction. 



Agar slant: Luxuriant, faintly yel- 

 lowish growth. 



Cellulose agar: Enzymatic zone 0.5 

 mm wide. 



Broth: Turbid. 



Litmus milk: Acid. 



Potato: Good growth. 



Indole not formed. 



Nitrites produced from nitrates. 



Ammonia is produced. 



Acid from glucose, fructose, arabinose, 

 xylose, maltose, lactose, sucrose, dextrin 

 and starch. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Compost from Arlington, Va. 



Habitat: Soil. 



24. Bacterium lucrosum McBeth. 

 (McBeth, Soil Science, 1, 1916, 461; 

 Cellulomonas lucrosa Bergey et al., 

 Manual, 1st ed., 1923, 167.) From Latin 

 lucrosus, lucrative. 



Rods: 0.4 by 1.3 microns. Non-mo- 

 tile. Gram-negative. 



Gelatin stab: No growth. 



Agar colonics: Circular, convex, semi- 

 transparent, granular, entire. 



Aga,r slant: Moderate, flat, grajash- 

 white growth, becoming somewhat iri- 

 descent. 



Ammonia cellulose agar: On crowded 

 plate, the colonies show an enzymatic 

 zone of 1 mm or more. 



Peptone cellulose agar: Enzymatic 

 zone 2 to 3 mm wide in 25 days. 



Broth: Turbid. 



Filter paper broth: Paper is reduced 

 to a grayish-white pulpy mass whose 

 fibers separate on slight agitation. 



Litmus milk: No change. 



Potato: No growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Ammonia not produced. 



Acid from glucose, maltose, lactose, 

 sucrose, starch and mannitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Soil from California. 



Habitat: Soil. 



25. Bacterium acidulimi Kellerman et 

 al. (Kellerman, McBeth, Scales and 

 Smith, Cent. f. Bakt., II Abt., 39, 1913, 

 513; Cellulomonas acidula Bergey et al.. 

 Manual, 1st ed., 1923, 167.) From Latin 

 acidus, acid. 



Rods : 0.3 by 1 .0 micron. Non-motile. 

 Gram-negative. 



