FAMILY BACTERlACEAE 



615 



Gelatin stab: No liquefaction. 



Agar slant: Slight, grayish growth. 



Cellulose agar: Enzymatic zone 0.5 to 

 1 mm in width. 



Broth: Clear. 



Litmus milk: Acid. 



Potato: Xo growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Ammonia not produced. 



Acid from glucose, maltose, lactose 

 and sucrose. None from gh^cerol or 

 mannitol. 



Starch not hydroh'zed. 



Aerobic. 



Optimum temperature 20°C. 



Source: Soil from Utah. 



Habitat: Soil. 



26. Bacterium castigatum ^IcBeth. 

 (McBeth, Soil Science, 1, 1916, 458; 

 Cellulomonas casligata Bergey et al., 

 Manual, 1st ed., 1923, 168.) From Latin 

 castigatus, subdued. 



Rods: 0.4 by 1.2 microns. Non-mo- 

 tile. Gram-negative. 



Gelatin stab: ^loderate surface 

 growth. No liquefaction. 



Agar colonies: Circular, slightly con- 

 vex, brittle, grajish-white, granular, 

 entire. 



Agar slant: Abundant, glistening, 

 grayish-white growth. 



Ammonia cellulose agar: Enzymatic 

 zone may attain a diameter of 2.5 mm in 

 30 days. 



Peptone cellulose agar: Enzj'matic 

 zone may reach a diameter of 2 mm in 

 30 days. 



Broth: Slightly turbid. 



Filter paper broth: Paper completely 

 disintegrated and reduced to a pulp-like 

 mass in 15 da vs. 



Litmus milk: Acid, not digested. 

 Indole not formed. 

 Nitrites not produced from nitrates. 

 Ammonia not produced. 

 Acid from glucose, maltose, lactose, 

 sucrose, starch and gh'cerol. 

 Aerobic. 



Optimum temperature 20°C. 

 Source: Soil from California. 

 Habitat: Soil. 



27. Bacteriimi bibulum (McBeth and 

 Scales) Holland. {Bacillus bibulus'Mc- 

 Beth and Scales, Bur. of Plant Industrj-, 

 U. S. Dept. of Agr., Bui. No. 266, 1913, 

 35; Holland, Jour. Bact., 5, 1920, 217 

 and 221; Cellulomonas bibula Berge}' et 

 al., Manual, 1st ed., 1923, 158.) From 

 Latin bibulus, thirsty. 



Rods: 0.4 by 1.3 microns. ^Motile. 

 Gram-negative. 



Gelatin stab : Crateriform liquefaction. 



Cellulose agar colonies: Circular, con- 

 vex, smooth, soft, grayish to faintly 

 yellowish-white, finely granular. En- 

 zymatic zone 0.3 mm in some cases. 



Agar slant: Luxuriant, glistening, 

 smooth, moist, raised growth. 



Broth: Slightly turbid. 



Litmus milk: Faintly acid. 



Potato: Smooth, glistening, canary 

 yellow growth. 



Indole is formed. 



Nitrites not produced from nitrates. 



Ammonia is produced. 



Acid from glucose, maltose, lactose, 

 sucrose, starch, glycerol and mannitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: From sewer slimes and culti- 

 vated soils. 



Habitat: Soil. 



Appendix I : The genus Cellulomonas as originally proposed was based on a single 

 physiological property and included such diverse types of bacteria as (1) polar 

 flagellate species, now placed in Pseudomonas, (2) Gram-variable, non-motile rods 

 now placed in Corynebacterium, and (3) peritrichous, non-spore-forming, Gram-nega- 

 tive rods. Unfortunately the name is unsuitable for the third of these groups so 

 that it has not been inserted in the outline used in this edition of the Manu.a.l. De- 

 scriptions of species previously placed in this genus are given below. 



