618 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Nitrites not produced from nitrates. 



Ammonia produced. 



Acid from glucose, maltose, lactose, 

 sucrose, starch and glycerol. No acid 

 from mannitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Soil from Louisiana. 



Habitat: Soil. 



4. Cellulomonas folia Sanborn. (Jour. 

 Bact., 1£, 1926, 1 and 343.) From Latin 

 folium, leaf. 



Description from Sanborn (Jour. 

 Bact., 18, 1929, 170) and also from his 

 unpublished notes. 



Rods: 0.8 to LO by 1.0 to L5 microns, 

 occurring singly and in short chains. 

 Motile with four to six peritrichous 

 flagella. Gram-negative. 



Gelatin stab: Slow crateriform lique- 

 faction, becoming stratiform. 



Agar slant: Growth moderate, dirty- 

 white, echinulate, raised, glistening, 

 opaque, butyrous. 



Broth: Turbid with yellowish sedi- 

 ment . 



Litmus milk: Alkaline. 



Potato: Thick, moist, yellowish-brown 

 growth . 



Indole not formed. 



Nitrites produced from nitrates. 



Acid and gas slowly produced from 

 glucose, sucrose, glycerol and mannitol 

 after prolonged incubation. No acid or 

 gas from lactose. 



Starch hydrolj^zed. 



Ammonia produced. 



No HoS formed. 



Aerobic, facultative. 



Optimum temperature 25° to 30°C. 



Resembles Cellulomonas rossica. 



.Source: From decomposing leaves. 



Habitat: Occurring in soil and active 

 in decomposing leaves in composts, hav- 

 ing the property of digesting cellulose. 



5. Cellulomonas flava Sack. (Cent. f. 

 Bakt., II Abt., 62, 1924, 79.) From 

 Latin flavus, yellow. 



Rods: 0.2 by 1.5 microns. Motile. 

 Gram-negative. 



Gelatin colonies: Circular, citron 

 yellow. 



Gelatin stab: Very slow liquefaction. 



Agar colonies: Large, circular, citron 

 yellow. 



Agar slant: Abundaiat, citron yellow 

 streak. 



Broth: Turbid with pellicle and sedi- 

 ment. 



Litmus milk: Unchanged. 



Potato: Light brown streak. 



Indole not formed. 



Nitrites and ammonia produced from 

 nitrates. 



Hydrogen sulfide produced. 



Cellulose hydrolyzed. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Habitat: Soil. 



6. Cellulomonas cellasea (Kellerman 

 et al.) Bergey et al. {Bacillus cellaseus 

 Kellerman, McBeth, Scales and Smith, 

 Cent. f. Bakt., II Abt., 39, 1913, 508; 

 Bergey et al., Manual, 1st ed., 1923, 158.) 



Rods: 0.5 by 1.2 microns. Motile 

 with one to three peritrichous flagella. 

 Gram-negative. 



Gelatin stab: Liquefaction. 



Agar slant: Limited grayish growth. 



Cellulose agar : Enzj^matic zone 0.5 mm 

 or less. 



Broth: Clear. 



Litmus milk: Acid. 



Potato: No growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Ammonia not produced. 



Acid from glucose, maltose, lactose, 

 sucrose, starch, glycerol and mannitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Soil from Utah. 



Habitat: Soil. 



7. Cellulomonas iugis (McBeth) Ber- 

 gey et al. (Bacillus iugis McBeth, Soil 

 Science, 1, 1916, 456; Bergey et al.. 

 Manual, Isted., 1923, 158.) From Latin, 

 joined together. 



Rods: 0.4 by 1.4 microns. Motile with 



