FAMILY BACTEKIACEAE 



619 



one to three peritrichous flagelki. 

 Gram-negative. 



Gelatin stab: Xapiform liquefaction. 



Agar colonies: Circular, convex, soft, 

 grayish-white, granular, entire. 



Agar slant: Scant, grayish-white, 

 filiform growth. 



Ammonia cellulose agar: After 20 days, 

 all colonies show an enzymatic zone of 

 1 mm or more. 



Peptone cellulose agar: Enzymatic 

 zone continues to increase up to 30 da3'S 

 at which time it may reach 5 mm in 

 width. 



Broth: Turbid. 



Filter paper broth: After 15 days, the 

 paper shows many ragged holes but dis- 

 integrates readily. 



Litmus milk: Acid, not digested. 



Potato: Abundant, glistening, gray- 

 ish-white growth. 



Indole not formed. 



Nitrites produced from nitrates. 



Ammonia produced. 



Acid from glucose, maltose, lactose, 

 sucrose, starch, glycerol and mannitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Soil from California. 



Habitat: Soil. 



8. Cellulomonas concitata (McBeth) 

 Bergey et al. {Bacillus concitatus Mc- 

 Beth, Soil Science, 1, 1916, 448; Bergey 

 et al.. Manual, 1st ed., 1923, 159.) From 

 Latin cuncitatus, rapid. 



Rods: 0.5 by 1.2 microns. Motile 

 with one to four peritrichous flagella. 

 Gram-negative. 



Gelatin stab: Xapiform liquefaction. 



Agar colonies: Irregularly circular, 

 decidedly convex, soft, becoming viscid, 

 grayish-white, sometimes slightly fluo- 

 rescent, granular, entire. 



Agar slant: Abundant, flat, moist, 

 faint yellowish-white growth. 



Ammonia cellulose agar: Surface col- 

 onies show an enzymatic zone of 1.0 to 

 1.5 mm. Deep colonies no zone but 

 colony somewhat clearer than surround- 

 ing medium. 



Peptone cellulose agar: Lnzjiuatic 

 zone, surface colonies, 2 to 2.5 mm; bot- 

 tom colonies, 1 mm or less. 



Broth: Turbid. 



Filter paper broth; In 15 days, the 

 paper is a disintegrated fibrous mass 

 which retains its pure white color. 



Litmus milk: Acid, not digested. 



Potato: No growth. 



Indole is formed. 



Nitrites not produced from nitrates. 



Ammonia not produced. 



Acid from fructose, maltose, lactose, 

 sucrose, starch and glycerol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Soil from California. 



Habitat: Soil. 



9. Cellulomonas caesia (Kellerman et 

 al.) Bergey et al. {Bacillus caesius 

 Kellerman, McBeth, Scales and Smith, 

 Cent. f. Bakt., II Abt., 39, 1913, 507; 

 Cellulomonas caseia (sic) Bergey et al., 

 Manual, 1st ed., 1923, 159; Cellulomonas 

 casei (sic) Bergey et al., Manual, 4th ed., 

 1934, 199.) From Latin caesius, bluish- 

 gray. 



Rods: 0.4 by 1.5 microns. Motile with 

 one or two peritrichous flagella. Gram- 

 negative. 



Gelatin stab: Liquefaction. 



Beef agar streak: Moderate, flat, thin 

 growth, slightly bluish fluorescence. 



Cellulose agar: Enz^-matic zone, 0.5 

 to 1.0 mm in 15 days. 



Broth: Turbid. Slight sediment in 

 5 days. 



Litmus milk: Acid, digested. 



Potato: No growth. 



Indole not formed. 



Nitrites produced from nitrates. 



Ammonia produced. 



Acid from glucose, maltose, lactose, 

 sucrose, starch, glycerol and mannitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Soil from Louisiana, Wiscon- 

 sin and New Hampshire. 



Habitat: Soil. 



