FAMILY BACTERIACEAE 



621 



Manual, 1st ed., 1923, 161.) From Latin 

 almus, nourishing. 



Rods : 0.5 by 1.2 microns. Motile with 

 one to five peritrichous flagella. Gram- 

 negative. 



Gelatin stab : Scant growth. Xo lique- 

 faction. 



Agar colonies: Circular, convex, soft, 

 becoming brittle, grayish-white, granu- 

 lar, entire. 



Ammonia cellulose agar: Enzj^matic 

 zone 3 to 4 mm in 25 days. 



Peptone cellulose agar: Enzymatic 

 zone 2.5 to 3.5 mm in 30 days. 



Agar slant: Scant, grayish-white 

 growth, becoming yellowish-white. 



Broth: Slightly turbid. 



Filter paper broth: Paper reduced to 

 a loose felt -like white mass in 15 days. 



Litmus milk: Slightly acid, not di- 

 gested. 



Potato: Xo growth. 



Indole not formed. 



Xitrites not produced from nitrates. 



Ammonia not produced. 



Acid from glucose, maltose, lactose, 

 sucrose, starch and glycerol. Xo acid 

 from mannitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Soil from California. 



Habitat: Soil. 



14. Cellulomonas desidiosa (McBeth) 

 Breed. {Bacillus desiduosus (sic) Mc- 

 Beth, Soil Science, 1, 1916, 450; Cellulo- 

 monas deciduosa (sic) Bergey et al., 

 Manual, 1st ed., 1923, 162; Breed, in 

 Manual, 5th ed., 1939, 495.) From 

 Latin desidiosus, inactive. 



Rods: 0.4 by 1.0 micron. Motile with 

 one to three peritrichous flagella. 

 Gram-negative. 



Gelatin stab: Moderate growth. Xo 

 liquefaction. 



Agar colonies: Circular, slightly con- 

 vex, soft, becoming somewhat viscid, 

 grayish-white, granular, entire. 



Agar slant: Scant, flat, grayish -white 

 growth . 



Ammonia cellulose agar: Enzymatic 

 zone 3 to 3.5 mm in 25 days. 



Peptone cellulose agar: Enzymatic 

 zone 1 to 2 mm around surface colonies. 

 Bottom colonies frequently show no en- 

 zymatic zone until after 20 days. 



Broth: Slightly turbid. 



Filter paper broth: Paper is divided 

 into gray white mass which readily dis- 

 integrates. 



Litmus milk: Acid, not digested. 



Potato: Xo growth. 



Indole is formed. 



Xitrites produced from nitrates. 



Ammonia not produced. 



Acid from glucose, lactose, maltose 

 and starch. X^o acid from mannitol, 

 sucrose or glycerol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Soil from California. 



Habitat: Soil. 



15. Cellulomonas pusilla (Kellerman 

 et al.) Bergey et al. {Bacillus pusilus 

 (sic) Kellerman, McBeth, Scales and 

 Smith, Cent. f. Bakt., II Abt., 39, 1913, 

 513; Cellulomonas pusila (sic) Bergey 

 et al.. Manual, 1st ed., 1923, 161.) From 

 Latin pusilla, very small. 



Rods: 0.6 by 1.1 microns. Motile 

 with one to three peritrichous flagella. 

 Gram -negative. 



Gelatin stab: Xo liquefaction. 



Agar slant: Scant, grayish -white 

 growth. 



Cellulose agar: Enzymatic zone i mm 

 or less in width. 



Broth: Turbid. 



Litmus milk: Acid. 



Potato: Xo growth. 



Indole not formed. 



Xitrites produced from nitrates. 



Ammonia is produced. 



Acid fi'om glucose, maltose, lactose, 

 sucrose, starch and glycerol. Xo acid 

 from mannitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



