622 



MANUAL OF DETERMTN'ATIYE BACTEUIOLOGY 



Source : Soil from District of Columbia 

 and South Carolina. 

 Habitat: Soil. 



16. Cellulomonas gelida (Kellerman et 

 al.) Bergey et al. (Bacillus gelidus 

 Kellerman, McBeth, Scales and Smith, 

 Cent. f. Bakt., II Abt., 39, 1913, 510; 

 Bergey et al., Manual, 1st ed., 1923, 162.) 

 From Latin gelidus, stiff. 



Rods: 0.4 by 1.2 microns. Motile 

 with one to three peritrichous flagella. 

 Gram -negative. 



Gelatin stab: No liquefaction. 



Agar slant: Lu.xuriant, grayish-white 

 growth. 



Cellulose agar: Enzymatic zone 1.5 

 mm in width. 



Broth: Turbid. 



Litmus milk: Acid, peptonized. 



Potato: Grows well. 



Indole not formed. 



Nitrites not produced from nitrates. 



Ammonia is produced. 



Acid from glucose, maltose, lactose, 

 sucrose, starch and glycerol. No acid 

 from mannitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: Soil from Connecticut. 



Habitat: Soil. 



17. Cellulomonas flavigena (Kellerman 

 and McBeth) Bergey et al. {Bacillus 

 flavigena Kellerman and McBeth, Cent, 

 f. Bakt., II Abt., 34, 1912, 488; Bergey 

 et al., Manual, 1st ed., 1923, 165.) From 

 Latin, yellow-producing. 



Rods: 0.4 by 1.0 micron. Motility 

 not recorded. Gram-negative. 



Gelatin stab: Liquefaction. 



Agar slant: Luxuriant, yellow growth. 



Cellulose agar: Enzymatic zone 0.75 

 to 1.5 mm in width. 



Broth: Turbid. 



Litmus milk: Acid. 



Potato: Grows well. 



Indole not formed. 



Nitrites produced from nitrates. 



Ammonia not produced. 



Acid from glucose, fructose, arabinose, 



xylose, maltose, lactose, sucrose, dextrin, 

 starch, inulin, salicin, glycerol and man- 

 nitol. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: From contaminated culture. 



Habitat: Soil. 



18. Cellulomonas rossica (Kellerman 

 and McBeth) Bergey et al. (Bacillus 

 rossicus Kellerman and McBeth, Cent, 

 f. Bakt., II Abt., 34, 1912, 492; Bergey 

 et al., Manual, 1st ed., 1923, 157; Proteus 

 cellulomonas var. Prote^is rossicus Prib- 

 ram, Klassifikation der Schizomyceten, 

 Leipzig und Wien, 1933, 72.) 



Rods: 0.3 by 1.2 microns. Motility 

 not recorded. Gram-negative. 



Gelatin stab: Rapid liciuefaction. 



Agar slant: Luxuriant, yellow growth. 



Cellulose agar: Enzymatic zone 0.5 to 

 1.0 mm in width. 



Broth: Turbid. 



Litmus milk: Alkaline. 



Potato: Grows well. 



Indole not formed. 



Nitrites produced from nitrates. 



Ammonia produced. 



No acid from carbohydrate media. 



Aerobic, facultative. 



Optimum temperature 20°C. 



Source: From contaminated culture. 



Habitat: Soil. 



Appendix II : Tlie following cellulose- 

 digesting bacteria are not included 

 above: 



Achromobacler picru7n Fuller and Nor- 

 man. (Jour. Bact., 46, 1943, 276.) 

 From soil. 



Bacillus aurogenes var. albus Keller- 

 man, McBeth, Scales and Smith. (Cent, 

 f. Bakt., II Abt., 39, 1913, 506.) From 

 soil from New York State. Differs from 

 Cellulomonas aurogenes in that it shows 

 no chromogenesis. 



Bacillus rossicus var. castaneus Keller- 

 man et al. (loc. cit., 508; Proteus cellu- 

 lomonas var. Proteus castaneus Pribram, 

 Klassifikation der Schizomyceten, Leip- 

 zig und Wien, 1933, 72.) From «oils 



