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MANUAL OF DETERMINATIVE BACTERIOLOGY 



0.5 by 2 microns, having a densely stain- 

 ing granule. The tapering pointed ends 

 remain unstained. Older cells have 

 rounded ends, are spherical, pear- 

 shaped or a long ellipsoid, stain weakly. 

 Non-motile. Gram-negative. 



No growth on ordinary media. See 

 preceding species. 



Medium S with pneumococcus I car- 

 bohydrate and 0.8 per cent agar: Very 

 tiny, pale yellow colonies. Less than 

 0.5 mm in diameter. 



Starch not hydrolyzed. 



Growth in sucrose broth. No growth 

 in glucose, lactose, maltosef dextrin, 

 inulin, mannitol and saliein broths. 



Aerobic. 



Minimum temperature 20°C. Opti- 

 mum 28° to 32°C. Maximum 34°C. 

 Thermal death point 48°C for 10 minutes. 



Minimum pH 6.0. Optimum pH 6.6 

 to 7.2. Maximum pH 7.8. 



Distinctive characters: Decomposes 

 the carbohydrate of pneumococcus 

 Type I. The addition to any favorable 

 medium of 0.7 per cent sodium chloride, 

 of 0.3 per cent beef extract or of 0.5 per 

 cent peptone completely inhibits growth. 



The composition of Medium S is as 

 follows: MgS07H20, 0.2 g, NH4H2PO4, 

 1.5 g, CaCl2, 0.1 g, FeCh, tr, KCl, 0.1 g, 

 10 cc N/1 NaOH. Distilled water 1,000 

 ml, pH 7.2 to 7.4. To this was added 

 the specific pneumococcus carbohydrate 

 as a source of carbon in concentrations 

 varying from 0.002 to 0.01 per cent. 



Source: From swamps and other un- 

 cultivated soils. 



Habitat: Soil. 



28. Bacterium nenckii Biernacki. 

 (Biernacki, Cent. f. Bakt., II Aht.,.29, 

 1911, 166; Achromobacter nenckii Bergey 

 et al.. Manual, 3rd ed., 1930, 227.) 

 Named for Nencki, a chemist at the 

 Medical Institute in Warsaw. 



Rods: 0.8 by 1.25 to 2.0 microns, with 

 rounded ends, occurring singly and 

 in pairs. Capsulated. Non-motile. 

 Gram-negative. 



Gelatin colonies: Circular, convex, 

 yellowish -white, granular. 



Glucose and sucrose gelatin: Colonies 

 larger and slimy. 



Gelatin stab: No liquefaction. 



Agar colonies : Circular, grayish -white, 

 glistening, concentric, finely granular. 



Agar slant: The medium is liquefied. 



Glucose and sucrose agar : Heavy slimy 

 growth with gas. Faint fruity odor. 



Broth: Slightly turbid with gray sedi- 

 ment and slight odor. 



Litmus milk: Acid and gas formation. 



Potato: Slight growth. 



Glycerol potato: Heavy growth with 

 the appearance and consistency of cream. 



Indole not formed. 



Nitrites not produced from nitrates. 



Acid and gas from glucose, fructose, 

 galactose, maltose, sucrose, raffinose and 

 mannitol. 



Fruity odor in cultures. 



Facultative anaerobe. 



Optimum temperature 37°C. 



Source: From Spanish dried grapes. 



Habitat: Unknown. 



29. Bacterium polysiphoniae Lunde- 

 stad. (Lundestad, Cent. f. Bakt., II 

 Abt., 75, 1928, 331; Flavobacterium poly- 

 siphoniae Bergey et al., Manual, 3rd 

 ed., 1930, 152.) From Greek, many 

 tubes. 



Rods: 0.5 to 0.6 by 2.0 to 4.0 microns, 

 with rounded ends, occurring singly. 

 Non-motile. Gram-negative. 



Fish-gelatin colonies: Circular, 

 slightly glistening, bright yellow, trans- 

 parent, with denser center. 



Fish-gelatin stab: Slight yellowish 

 growth on surface. Slow saccate lique- 

 faction. 



Sea-weed agar colonies: Circular, flat, 

 with concentric rings, diffuse margin, 

 light yellow. Agar is disintegrated. 



Fish-agar slant: Yellow, flat growth, 

 with undulate margin. 



Broth : Turbid with flocculent pellicle 

 and yellowish sediment. 



Indole not formed. 



