630 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



Source: Marine algae; isolated from 

 NercGcystis luetkeana. 

 Habitat: On marine algae. 



6. Agarbacterium aurantiaciun Angst. 

 (Puget Sound Biol. Sta. Pub., 7, 1929, 

 49.) 



Short rods with rounded ends, 0.6 to 

 0.8 micron, occurring singly or in pairs. 

 Motile. No capsules. Gram-negative. 



Fish gelatin colonies : Circular, sunken, 

 crateriform, entire. 



Fish gelatin stab: Stratiform liquefac- 

 tion, no growth along line of stab. 



Fish agar slant: Abundant, filiform, 

 flat, glistening, smooth, opaque, orange, 

 butyrous growth. 



Fish agar colonies: Circular, smooth. 

 Hat, erose, sunken, granular. 



Digests agar; cellulose not attacked. 



Fish broth: Membranous pellicle, tur- 

 l)id, no sediment. 



Plain milk unchanged; surface growth 

 orange. 



Potato: Alumdant, filiform, flat, dull, 

 smooth, orange, butyrous growth. 



Nitrites not produced from nitrates. 



No HoS or indole formed. 



Acid from lactose and mannitol. No 

 acid from xylose, rhamnose, arabinose, 

 glucose or sucrose. 



Starch not hydrolyzed. 



Aerobic. 



Optimum temperature 20° to 28°C; 

 thermosensitive. 



Source: Isolated from Porphi/ra per- 

 forata. 



Habitat: On marine algae. 



7. Agarbacterixim cyanoides Angst. 

 (Puget Sound Biol. Sta. Pub., 7, 1929, 

 49.) 



Short rods with rounded ends, 0.8 by 

 1.4 microns, occurring singly or in pairs. 

 Motile. No capsules. Gram-negative. 



Fish gelatin colonies : Circular, sunken , 

 entire, crateriform, granular. 



Fish gelatin stab: Stratiform liquefac- 

 tion, growth only at top. 



Fish agar slant: Abundant, filiform, 

 raised, glistening, smooth, opaque, gray, 

 butyrous growth. 



Fish agar colonies: Circular, smooth, 

 flat, lobed, granular, greenish to yel- 

 lowish. 



Digests agar; cellulose not attacked. 



Fish broth : Flocculent pellicle, turbid, 

 no sediment, fluorescent green. 



Potato: Abundant, filiform, raised, 

 glistening, smooth, buff, butyrous 

 growth. 



Nitrites not produced from nitrates. 



No HiS or indole formed. 



Plain milk acidified, greenish surface 

 growth. 



Acid from sucrose. No acid from xy- 

 lose, arabinose, glucose, lactose, manni- 

 tol or rhamnose. 



Starch is hydrolyzed. 



Aerobic. 



Optimum temperature 20° to 28°C; 

 thermosensitive. 



Source: Isolated from Iridaea curdata. 



Habitat : On marine algae. 



Note: Seven additional species are 

 described with as much detail by Angst 

 {loc. cit.) as are the six above species; 

 but he refers to them only as Agarbac- 

 terium Nos. 5, 6, 7, 8, 9, 13, 14, and 15. 

 All digest agar. 



8. Flavobacterium uliginosum ZoBell 

 and Upham. (Bull. Scripps Inst, of 

 Oceanography, Univ. Calif., 5, 1944, 

 2G3.) From Latin uligo, ooze or moist 

 mud. 



Rods: 0.4 to 0.6 by 1.2 to 3.9 microns, 

 some slightly curved, occurring mostlj^ 

 singly with some short chains. Non- 

 motile. Gram-negative. 



All differential media except the fresh- 

 water broth, litmus milk, and potato 

 were prepared with sea water. 



Gelatin colonies: 1 mm, orange, 

 sunken. 



Gelatin stab: Infundibuliform lique- 

 faction. Yellow pigment. Gelatin dis- 

 colored brown. 



Agar colonies : Sunken, uneven, irregu- 

 lar, gummy colonies which liciuefy agar. 

 Produces orange to yellow pigment and 

 discolors agar brown. 



Agar slant: Luxuriant, yellowish- 



