FAMILY BACTERIACEAE 



631 



orange, glistening, filiform, adherent 

 growth which slowh' liquefies agar. 



Sea-water broth : Dense yellow pellicle, 

 moderate turbidity, slightly viscid sedi- 

 ment. 



Fresh-water broth: Xo visible growth. 



Litmus milk: Completelj' decolorized, 

 neutral. 



Potato: Xo visible growth. 



Indole not formed. 



X'itrates rapidly reduced to nitrites. 



Produces acid but no gas from xylose, 

 glucose, maltose, lactose, sucrose and 

 salicin. Does not ferment glycerol or 

 mannitol. 



Starch not hydrolyzed. 



Hydrogen sulfide not formed. 



Ammonia produced from peptone but 

 not from urea. 



Casein digested. 



Fats not hydrolyzed. 



Agar liquefied rapidly. However, 

 after prolonged laboratory cultivation 

 this organism gradually loses its ability 

 to digest agar. 



Aerobic, obligate. 



Optimum temperature 20° to 25°C. 



Source: jNIarine bottom deposits. 



9. Flavobacterium amocontactum Zo- 

 Bell and Allen. (Jour. Bact., 29, 1935, 

 246.) From Latin amo, to love and con- 

 tactus, touching, contacting. 



Slender rods: 0.4 to 0.7 by L6 to 2.3 

 microns, with rounded ends, occurring 

 singly and in irregular clumps. Stain 

 very lightly. Possess well-defined cap- 

 sules. Actively motile by means of peri- 

 trichous flagella. Gram-negative. 



Gelatin stab: Good filiform growth 

 with rapid saccate liquefaction. 



Agar colonies: Circular. 2.0 to 4.0 mm 

 in diameter, yellow. 



Agar slant: Abundant, filiform, 

 smooth, glistening, abundant, bright 

 yellow growth having a but3'rous con- 

 sistency. Originally liquefied agar, but 

 this propertj^ was lost following artificial 

 cultivation. 



Sea water broth: Good growth with 

 ring at surface. Strong turbidity and 

 abundant viscid sediment. Xo odor. 



^lilk: Xo growth. 



Potato: Xo growth. 



Potato dialyzed in sea water: Slight 

 yellow growth. 



Lidole not formed. 



Xltrites produced from nitrates. 



Ammonia liberated from peptone. 



Hydrogen sulfide produced. 



Xo acid from glucose, lactose, sucrose, 

 xylose or mannitol. 



Starch not attacked. 



Optimum reaction pH 8.0. 



Optimum temperature 18° to 21°C. 



Facultative aerobe. 



Distinctive character: Adheres firmly 

 to submerged glass slides; cannot be 

 removed with running water. 



Source: Many cultures isolated from 

 glass slides submerged in sea water. 



Habitat: Sea water. 



Appendix II: Another species de- 

 scribed recently is: 



Bacillus exedens Wieringa. (Wier- 

 inga, Jour. Microbiol, and Serol., 7, 

 1941, 121; Bacillus agar-exedens Wier- 

 inga, idem.) From stable manure, leaf- 

 mold and soil. Liquefies agar. 



37. Bacterium chitinophilum Hock. 

 (Jour. [Marine Res., 4, 1941, 103.) From 

 ^I. L., chitin and Greek philos, loving. 



Short rods: 0.35 to 0.65 by 0.95 to 1.5 

 microns. Motile. Gram-negative. 



Sea water gelatin: Liquefaction; 

 growth absent in stab but abundant in 

 liquefied zone. 



Sea water agar plate: Colonies circu- 

 lar, smooth, entire, raised, white. 



Sea water liquid medium: Moderate 

 growth, sometimes with formation of 

 ring or pellicle. Scant granular sedi- 

 ment. 



Decomposes natural chitinous material 

 such as horseshoe crab shells and also 

 purified chitin. 



Four out of five strains produce ni- 

 trites from nitrates. 



Acid from glucose and usually from 

 sucrose, glycerol and mannitol. One of 

 five cultures produced acid from lactose. 

 Does not digest cellulose. 



