642 



MANUAL OF DETERMINATIVE BACTERIOLOGY 



very limited turbidity; no pellicle; no 

 sediment. 



Litmus milk containing salt: No ap- 

 parent growth. 



Potato moistened with sea water: 

 Moist, spreading growth, cream-colored; 

 heavy sediment in free liquid at bottom. 



Starch plate: Limited, pale blue 

 growth; no di9,stase. 



Aerobic. 



Optimum temperature 20°C. 



Source: From sea water, and from the 

 surface of algal growth. 



Habitat: Common in sea water. 



52. Bacterium terrestralginicum Waks- 

 man et al. (Waksman, Carey and 

 Allen, Jour. Bact., 28, 1934, 217.) 



Long rods, with somewhat rounded 

 ends, usually single, but also in pairs, 

 and occasionally in chains of shorter 

 rods. 1.0 to 1.5 by 1.5 to 2.5 microns. 

 Motile. Granular. Gram-negative. 



Alginic acid plate: Colonies small, 

 whitish in appearance with a slight 

 metallic sheen. 



Alginic acid liquid medium: Medium 

 at first clouded. Later, a pellicle is 

 formed on the surface of the medium, 

 which is soon broken up due to active 

 gas formation. Reaction of medium 

 becomes slightly alkaline. 



Gelatin medium : Slow growth through- 

 out stab, slow liquefaction at surface 

 of medium at 18°C. 



Agar liquefaction: None. 



Glucose broth: Abundant turbidity, 

 some sediment, no pellicle, slightly 

 fluorescent. 



Litmus milk: Acid, milk coagulated, 

 only limited digestion of coagulum. 



Potato: Abundant, pinkish, compact, 

 dry growth on surface of plug, the rest 

 of plug becoming gray, with a tendency 

 to darkening. 



Starch plate: Limited growth along 

 streak, no diastase. 



Aerobic to facultative anaerobic. 



Optimum temperature 30°C. 



Source: From New Jersey soil. 



Habitat: Soil. 



53. Bacteriimi cygni Migula. (Septi- 

 kamiebacillus der Schwane, Fiorentini, 

 Cent. f. Bakt., 19, 1896, 935; Migula, 

 Syst. d. Bakt., 2, 1900, 365; Bacillus 

 cygneus Chester, Manual Determ. Bact., 

 1901, 221.) From Latin cygnus, swan. 



Rods: Motile. Gram-negative. 



This organism may have been the 

 fowl cholera or septicemia organism 

 (Pasteurella avicida Trevisan); but. is 

 more probably closely related to the 

 organism which causes keel in ducklings 

 {Salmonella anatis Rettger and Scoville). 



Source: From a swan. 



Habitat: The cause of an infectious 

 disease of swans in the city park at 

 Milan, Italy in 1895. 



54. Bacterium cyprinicida Plehn. 

 (Plehn, Cent. f. Bakt., I Abt., Orig., 

 35, 1903-04, 461; Klebsiella cyprinicida 

 Bergey et al., Manual, 2nd ed., 1925, 

 266.) From Greek kyprmos, carp and 

 Latin caedo, to kill. 



Rods: 0.8 by 1.0 micron, occurring 

 singly and in chains. Capsulated. 

 Non-motile. Gram-negative. 



Gelatin colonies: White, glistening, 

 conve.x, with slight fluorescence around 

 the colony in three or four days. 



Gelatin stab: White, convex surface 

 growth. No liquefaction. 



Agar slant: White, glistening layer, 

 becoming slimy. 



Broth: Turbid, with thick gray pellicle 

 and slimy sediment. 



Litmus milk: Slightly alkaline. No 

 coagulation. 



Potato: Light yellowish layer, be- 

 coming dark brownish. The medium is 

 dark violet-gray. 



Indole not formed. 



Nitrites not produced from nitrates. 



No acid from carbohydrate media. 



Aerobic, facultative. 



Optimum temperature 10° to 20°C. 



Habitat: The cause of a fatal disease 

 in carp, showing as red spots on the 

 ventral surface. 



55. Bacteriiunparvulum Conn. (N.Y. 



