FA^^LY BACTERIACEAE 



643 



Agr. Exp. Sta. Bui. -194, 1922,26.) From 

 Latin, very small. 



Very small rods: 0.1 to 0.2 by 0.3 to 

 0.5 micron. Xon-motile. Gram-nega- 

 tive. 



Gelatin plate: Punctiform colonies. 



Agar plate: Punctiform colonies. 



Grows poorly in liquid media. 



Indole not formed. 



Nitrites produced from nitrates. 



No acid from glucose, lactose, sucrose, 

 glycerol or ethyl alcohol in either liquid 

 or solid media. 



Starch not digested. 



Optimum temperature 25°C. 



Stricth' aerobic. 



Distinctive character: Causes strong 

 volatilization of ammonia from a mix- 

 ture of horse feces and urine. 



Source: From manure. 



Habitat: Soil. 



56. Bacteriiun methylicum (Loew) Mig- 

 ula. {Bacillus methylicus Loew, Cent, 

 f. Bakt., 12, 1892, 465; Migula, Syst. d. 

 Bakt., 2, 1900, 447.) From the chemical 

 term, methyl. 



Short, thick rods: 1.0 by 2.0 to 2.5 

 microns. Gram stain not recorded. 



Gelatin colonies: After 2 days, round 

 to oval, yellowish, entire; later edges 

 ciliate. Liquefaction. 



Glucose gelatin stab: Liquefaction 

 crateriform. Whitish-yellowish sedi- 

 ment. No liquefaction in depth. 



Glucose gelatin stab: In depth, little 

 or no growth, slowly liquefied near 

 surface. 



Agar stab: Surface growth spreading, 

 grayish -white. No growth in depth. 



Broth: No turbidity. On the surface 

 and adherent to the walls, a white ring 

 which precipitates on shaking. 



Potato: Growth very slow, pure white, 

 adherent. 



Grows well in 0.5 per cent methyl 

 alcohol, 0.05 per cent di calcium phos- 

 phate, and 0.01 per cent magnesium sul- 

 fate, on which broth it forms a reddish 

 pellicle. 



Possesses the ability to decompose 

 formaldehyde and formic acid salts with 

 formation of a reddish pellicle. 



Aerobe. 



Source: A culture contamination from 

 the air. 



Habitat: Probably soil. 



Appendix I: A few of the numerous Gram-negative, motile or non-motile, non- 

 spore-forming rods that do not belong in the groups previously listed in this genus 

 are described here. All have been placed in the genus Bacillus by those who have 

 described them, although none form spores. 



I. Produce a pink to red chromogenesis. 



A. Motile. 



1. Bacillus lactorubefaciens. 



B. Non-motile. 



1. Gelatin liquefied. 



2. Bacillus rubricus. 



3. Bacillus rujus. 



2. Gelatin not liquefied. 



a. Salmon pink on agar. 



4. Bacillus mycoides corallinus. 

 aa. Vinous red on agar. 



5. Bacillus bruntzii. 



II. Produces a water-soluble orange to emerald green pigment. 

 A. Motile. 



1. Gelatin liquefied. 



6. Bacillus aurantiacus tingitanus 



