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MANUAL OF DETERMINATIVE BACTERIOLOGY 



1. Bacillus lactorubefaciens Gruber. 

 (Gruber, Cent. f. Bakt., II Abt., 8, 

 1902, 457; Serralia lactorubefaciens Ber- 

 gey et al., Manual, 1st ed., 1923, 92.) 

 From Latin, to make milk red. 



Small rods: 0.4 to 0.6 by 3.5 microns, 

 occurring singly and in pairs. Motile 

 with peritriclious flagella. Gram reac- 

 tion not given. 



Gelatin colonies: Grayish-white, 

 smooth, glistening, spreading. 



Gelatin stab: At times arborescent; 

 the medium tinged with red. No lique- 

 faction. 



Agar colonies : Circular, lobed, grayish, 

 contoured. 



Agar slant: White, spreading growth. 



Broth: Turbid, with grayish pellicle 

 and slimy sediment. 



Limus milk: Becomes rose red, slimy, 

 slightly acid, without coagulation. 



Potato: White, spreading growth. 



No gas from carbohydrate media. 



Indole not produced. 



Nitrites produced from nitrates. 



Aerobic, facultative. 



Optimum temperature 25°C. 



Habitat: Milk. 



2. Bacillus rubricus Hefferan. (Hef- 

 feran. Cent. f. Bakt., II Abt., 11, 1903, 

 403; Erythrohacillus rubricus Holland, 

 Jour. Bact., 5, 1920, 220; Serralia rubrica 

 Bergey et al.. Manual, 1st ed., 1923, 313; 

 Chromobacterium rubricum Topley and 

 Wilson, Princ. Bact. and Immun., 7, 

 1931, 402.) 



Rods: 0.7 to 0.9 by 1.0 to 4.0 microns, 

 occurring singly. Non-motile. Gram 

 reaction not» given. 



Gelatin colonies: Small, circular, yel- 

 low-orange, deepening to red. 



Gelatin stab: Slow liquefaction. Old 

 cultures lose this property. 



Agar colonies: Circular, raised, entire. 



Agar slant: Moist, spreading, white to 

 pink, gradually deepening in color. 



Broth: Turbid, with viscid sediment. 



Litmus milk: Alkaline. 



Potato: Slight growth, blight pink, 

 turning coral red. 



Indole not produced. 



Nitrites not produced from nitrates. 



No acid or gas from carbohydrate 

 media. 



Aerobic, facultative. 



Optimum temperature 25° to 30°C. 

 No growth at 37 °C. 



Source: Isolated from Mississippi 

 river water, also from buttermilk. 



3. Bacillus rufus Hefferan. (Hef- 

 feran, Cent. f. Bakt., II Abt., 11, 1903, 

 313; Erythrobacillus rufus Holland, Jour. 

 Bact., 5, 1920, 220; Serralia rufa Bergey 

 et al., Manual, 1st ed., 1923, 95.) From 

 Latin rufus, red. 



Differs from Bacillus rubricus in show- 

 ing more luxuriant growth on potato and 

 slower action in milk. 



Source: From Mississippi Uiver water. 



4. Bacillus mycoides corallinus Hef- 

 feran. (Hefferan, Cent. f. Bakt., II 

 Abt., 11, 1903, 459; Serralia corallina 

 Bergey et al., Manual, 1st ed., 1923, 93.) 



Small, slender rods: 1.2 to 2.0 microns 

 in length, occurring singly and in pairs. 

 Non-motile. Gram reaction not given. 



Gelatin colonies: Minute, becoming 

 pink, smooth, raised. 



Gelatin stab: Slow growth. Raised, 

 smooth, glistening, pink surface growth. 

 Fine, feathery growth in stab. No lique- 

 faction. 



Agar colonies: Minute, with filamen- 

 tous margin. 



Agar slant: Smooth, moist, salmon 

 pink. 



Broth; Turbid, with pink flakes on 

 surface. 



Litmus milk: Alkaline, with red sur- 

 face. 



Potato: Like agar slant. 



Indole not formed. 



Nitrites produced from nitrates. 



No gas from carbohydrate media. 



Aerobic, facultative. 



Optimum temperature 25° to 30°C. 



Source: Isolated from Mississippi 

 river water. 



5. Bacillus bruntzii Nepveux. (Nep- 

 veux, Compt. rend. Soc. Biol., Paris, 

 72, 1920, 242; These, Fac. de Pharm., 

 Jouve et Cie, Paris, 1920. 136 pp.; 

 Serralia bruntzii Bergey et al., Manual, 



