FAMILY BACTERIACEAE 



645 



3rd ed., 1930, 125.) Xamcd for Prof. 

 Bruntz of Paris. 



Bacillus roseus fluorescens Mar- 

 chal (Trav. Lab. Microbiol. Fac. 

 Pharm. Xanc}-, 1937, 90) is regarded by 

 Lasseur (personal communication, 1938) 

 as identical with Bacillus hruntzii 

 Xepveux. 



Rods: 0.3 to 0.5 by 1.25 to 1.5 microns, 

 occurring singly and in pairs. Xon- 

 motile. Gram-negative. The cells 

 store volutin and glycogen as reserve 

 materials. 



Gelatin colonies: Circular, gray, 

 smooth, contoured, glistening, undulate 

 margin, becoming red. 



Gelatin stab: Xo liquefaction. 



Agar colonies: Circular, flat, smooth, 

 contoured, radiate margin, vinous red. 



Agar slant: Smooth, echinulate, bu- 

 tyrous, vinous red in color. 



Broth: Turbid. 



Litmus milk: Unchanged. 



Indole not formed. 



Xitrites produced from nitrates. 



Acid from glucose, fructose, maltose, 

 lactose, sucrose, mannitol, dulcitol and 

 glycerol. 



Aerobic, facultative. 



Optimum temperature 20° to 25°C. 



Habitat: Water. 



6. Bacillus aurantiacus tingitanus 

 Remlinger and Bailly. (Compt. rend. 

 Soc. Biol. Paris, 119, 1935, 246.) 



Short rods: Usually 2 to 3 microns, 

 sometimes 5 to 6 microns long. Activeh^ 

 motile. Gram-negative. 



Growth occurs on all the ordinary 

 nutrient media. Fluorescent bright 

 orange pigment. 



Gelatin: Rapid liquefaction. 



Milk: Slow coagulation. 



Synthetic broth: Lasseur, Dupaix- 

 Lasseur and jNIarion (Trav. Lab. Micro- 

 biol. Fac. Pharm. Xancy, Fasc. 9, 1936, 

 34) recognize two rough types of this 

 organism, one of which forms a smooth 

 and the other a wrinkled pellicle. The 

 smooth type gives a rough (pH 4.7) or a 

 smooth (pH 6.3) pellicle according to the 

 pH of the medium. 



Indole not formed. 



Artichoke media: Luxuriant growth. 

 Emerald green pigment produced. On 

 transferring the culture to potato, the 

 bright orange pigment reappears. 



Coagulated serum: Xo liquefaction. 



Acid from sucrose, lactose, glucose, 

 mannitol and maltose. 



Xon -pathogenic. 



Optimum pH 6.6. Xo growth at pH 

 6.2, but grows at pH 7.8. 



Optimum temperature 20°C. Good 

 growth from 15° to 37 °C. 



Aerobic. 



Pigment: Orange or capucine pigment 

 which diffuses throughout the medium. 

 Xot affected by the presence or absence 

 of light. Pigment production depends 

 on the growth of the culture, not on the 

 acidity of the medium. Insoluble in 

 acetone, amyl alcohol and gasoline. 

 Partially soluble in ether and ethyl 

 alcohol which are colored yellow. 



Distinctive character: A fluorescent 

 pigment of an unusual shade (bright 

 orange). 



Source: From water at Tangiers. 



Habitat: Presumably water. 



Appendix II :* The anaerobic genus Methanobacterium was proposed tentatively 

 by Kluyver and Van Xiel in 1936 with indication that they regarded Sohngen's meth- 

 ane bacterium as the type species of the genus. Later, Barker (1936) found or- 

 ganisms that he regarded as identical with those previously isolated by Sohngen and 

 he proposed the name Methanobacterium sohngenii for this species. A second species 

 found at the same time was named Methanobacterium omelianskii and it was identified 

 as the species previously described but not named by Omeliansky. At the time, he 

 felt that these anaerobes should be included in the family Mycobacteriaceae (1936, 



* The manuscript for this section has been reviewed by Dr. H. Albert Barker, 

 University of California, Berkeley, California, February, 1945. 



