FAMILY BACILLACEAE 707 



9. Bacillus polymyxa. 

 aa. Acetylmethylcarbinol not produced. Crystalline dextrins formed from 

 starch. 



10. Bacillus viacerans. 

 2. No visible gas from carbohydrates. 



a. Saprophytic. Growth on ordinary media. 



b. pH of glucose, proteose-peptone broth cultures less than 8.0. 

 Citrates not used as source of carbon, 

 c. Starch hydrolyzed. Acid from sucrose with ammoniacal 

 nitrogen. 



d. Acid from xylose or arabinose with ammoniacal nitrogen. 

 Acetylmethylcarbinol not formed. 



11. Bacillus circulans. 



dd. No acid from xylose or arabinose. Acetylmethylcarbinol 

 produced. 



12. Bacillus alvei. 



cc. Starch not hydrolyzed. No acid from sucrose. 



13. Bacillus laterosporus. 



bb. pH of glucose, proteose-peptone broth cultures 8.0 or higher. 

 Citrates used as source of carbon. 



14. Bacillus brevis. 

 aa. Parasitic. No growth on ordinary media. 



b. Cause of American foul brood of honey bees. 



15. Bacillus larvae. 



bb. Cause of the milky disease of Japanese beetles {PopilHa japonica 

 ^ewm . ) . 



Type A. 16. Bacillus popilliae. 

 Type B. 17. Bacillus lentimorbus . 

 C. Spores spherical, central to terminal. Sporangia definitely bulged, drum- 

 sticks and spindles. Carbohydrates not attacked. Gram -variable. 



1. Growth on nutrient agar without urea or free ammonia. 



a. Urease not formed. 



18. Bacillus sphaericus. 

 aa. Urease produced. 



18a. Bacillus sphaericus var. fusiformis. 



2. No growth on nutrient agar without urea or free ammonia. Urease formed. 



19. Bacillus pasteurii. 



II. Thermophilic, optimum temperatures 55°C or above ; slight if any growth at 37°C. 

 Aerobic . * 

 A. Spores ellipsoidal to cylindrical, central to terminal; sporangia not distinctly 

 bulged. 

 1.. Diameter of rods less than 0.8 micron, 

 a. Gas from carbohydrates. 



20. Bacillus thermoamylolyticus. 

 aa. No gas from carbohydrates. 



* The data on the species of this group are so meager that it is not possible to offer a 

 rational system of classification. Many of the characters used for separating the vari- 

 ous species are probably as variable in this group as they have been found to be in the 

 mesophilic group. Lacking a knowledge of the limits of variability and lacking other 

 pertinent data, the present arrangement is regarded as temporary only. 



