708 MANUAL OF DETERMIXATIVE BACTERIOLOGY 



b. Growth below 50°C. 



c. Nitrites from nitrates, often with liberation of nitrogen gas. 



21. Bacillus kaustophilus. 

 21a. Bacillus pepo. 



cc. No nitrites from nitrates. 



22. Bacillus thennoindifferens. 

 bb. No growth below 50°C. 



23. Bacillus thermodiastaticus. 

 2. Diameter of rods greater than 0.8 micron. 



a. Growth on nutrient agar. 



b. Remnants of sporangium adherent. 



24. Bacillus cylindricus. 

 bb. Remnants of sporangium not adherent. 



25. Bacillus robustus. 

 25a. Bacillus losanitchii. 



aa. No growth on nutrient agar. 



26. Bacillus calidolaclis. 



B. Spores ellijjsoidal to cylindrical, central to terminal; sporangia distinctly 



bulged. 



1. Diameter of rods less than 0.9 micron. 



a. Starch hydrolyzed. 



b. Nitrites from nitrates, sometimes with liberation of nitrogen gas. 



27. Bacillus michaelisii. 

 27a. Bacillus lobatus. 



27b. Bacillus thermononliquefaciens. 

 bb. No nitrites from nitrates. 



c. Action on cellulose not recorded. 



28. Bacillus thermotranslucens. 

 28a. Bacillus stearothermophilus . 

 28b. Bacillus aerothermophilus. 



cc. Cellulose hydrolyzed. 



29. Bacillus thennocellulolyticus. 

 aa. Starch not hydrolyzed. 



b. Nitrites from nitrates, sometimes with gaseous nitrogen, 

 c. Milk unchanged. 



30. Bacillus thennoalimentophilus. 

 cc. Milk acid, coagulated. 



31. Bacillus thermoliquefaciens. 



2. Diameter of rods greater than 0.9 micron. 



a. Starch hydrolyzed. 



b. No nitrites from nitrates. 



32. Bacillus tostus. 



C. Spores spherical, central to terminal; sporangium not distinctly bulged. 



33. Bacillus viridulus. 



1. Bacillus subtilis Cohn emend. Praz- schichte und Fermentwirkung einigen 



mowski. (Cohn, Beitr. z. Biol. d. Pflan- Bakterien-Arten. Inaug. Diss., Leipzig, 



zen,;,Heft2, 1872, 174; Heft 3, 1875, 188; 1880.) From Latin subtilis, thin, 



2, Heft 2, 1876, 249; Prazmowski, Unter- slender, 



suchungen iiber die Entwicklungsge- The identity of this species has been 



