730 



MANUAL OF DETERMINATIVE BACTERIOLOGY' 



Rods: 0.6 by 5 to 8 microns. Motile. 

 Gram-positive. 



Gelatin stab : No liquefaction. 



Agar colonies: At 60°C of two types, 

 large and small, circular, translucent, 

 granular, slimy. 



Broth: Very weak growth, no surface 

 growth, no sediment. 



Milk: Not coagulated, slowly pepton- 

 ized. 



Potato : Slight growth. 



Nitrites produced from nitrates. 



Starch actively hydrolyzed. 



Acid and gas from glucose, fructose, 

 galactose, maltose, dextrin, starch and 

 glycerol. Arabinose, xylose and manni- 

 tol not fermented 



Thermophilic, optimum temperature 

 50° to 55°C. 



Aerobic. 



Source: From sewage. 



Habitat : Probably in decaying matter. 



21. Bacillus kaustophilus Prickett. 

 (N. Y. Agr. Exp. Sta. Tech. Bui. 147, 

 1928, 38.) From Greek kaustos, burnt, 

 red-hot; philos, loving; heat-loving. 



Spores: Ellipsoidal, 0..'3 by 0.6 to 0.8 

 micron, terminal to subterminal. Xo 

 free spores observed. 



Sporangia: Only slightly swollen if at 

 all. 



Rods: On yeast extract -nutrient agar 

 at 56°C, 0.7 by 2.0 to 4.5 microns, with 

 rounded ends. Actively motile. Gram- 

 positive. 



Gelatin stab: No growth at 20°C. 

 Liquefied at 56°C. 



Agar colonies: At 56°C, circular, con- 

 vex, smooth. Borders entire to irregular. 

 Show curled structure, strands of chains. 

 Brown by transmitted light. 



Agar slant : Growth abundant, raised, 

 glistening, contoured, bluish-green to 

 bluish-white by transmitted light. After 

 three weeks at 37°C, growth has a distinct 

 reddish-brown color, butyrous, viscid. 



Broth: Slightly turbid, no sediment. 

 No surface growth, alkaline. 



Litmus milk: Rennet coagulum, pep- 

 tonization feeble, litmus reduced. 



Potato : Amount of growth Variable, 

 brownish, spreading, glistening, slimy. 

 Some strains do not grow. 



Nitrites produced from nitrates, often 

 with the production of nitrogen. 



Starch is hydrolyzed. 



Acid from glucose and salicin. Rham- 

 nose, maltose, sucrose, raffinose, manni- 

 tol, sorbitol and inulin not fermented. 



Acetylmethylcarbinol not produced. 



Thermophilic, optimum temperature 

 60°C to 65°C. Growth at 73°C to 75°C 

 but none at 80°C on agar slants. 



Aerobic, facultative. 



Good growth occurs in synthetic media 

 containing potassium nitrate, sodium 

 ammonium phosphate, aspartic acid, and 

 sodium asparaginate, respectively, as 

 only sources of nitrogen with glucose as 

 source of carbon. 



Source : Forty -eight cultures isolated 

 from pastQurized milk at a single milk 

 plant (Buffalo, N.Y.). 



Habitat : Probably originally from soil 

 and dust. 



Thermobacillus digestans Feirer (Soil 

 Sci., 23, 1927, 50) seems to be closely 

 related to the preceding. It is more 

 strongly proteolytic, digesting milk com- 

 pletely in 7 days. 



Source : Four strains isolated from soil. 



21a. Bacillus pepo Shaw. (Jour. Inf. 

 Dis., 43, 1928, 473.) From Cucurbita 

 pepo, the pumpkin. 



From the brief original description, 

 this organism seems to vary from Bacillus 

 kaustophihts only in its distinctive viscid 

 or slimy character. 



Source: Two cultures isolated from 

 swelled cans of pumpkin. 



Habitat : Probably found in soil and 

 dust. 



22. Bacillus thermoindiflferens Wein- 

 zirl. (Jour. Med. Research, 39, 1919, 

 402.) From Greek thermos, hot and 

 Latin indifferens, indifferent. Indiffer- 

 ent to or tolerant of heat. 



Spores: Ellipsoidal, 0.5 by 0.8 micron, 

 terminal. 



