FAMILY BACILLACEAE 



731 



Sporangia: Cylindrical, not swollen. 



Rods : 0.7 by 2.0 to 4.5 microns, occur- 

 ring singh- and in short chains, with 

 rounded ends. ^lotile. Gram-positive. 



Gelatin stab: Growth filiform. Slow 

 infundibuliform liquefaction. 



Agar colonies : Circular, convex, 

 smooth, entire, amorphous. 



Agar slant: Growth flat, spreading, 

 glistening, translucent, butyrous, con- 

 toured . 



Broth: Turbid, abundant sediment. 

 Xo surface growth. 



Litmus milk: Alkaline. Litmus 

 reduced. 



Potato : No growth. 



Indole not formed. 



Nitrites not produced from nitrates. 



Starch is hydrolyzed. 



Acid froni glucose. No acid from 

 lactose, sucrose or mannitol. 



Thermophilic, optimum temperature 

 55°C. Grows at 20° to 37°C. 



Aerobic . 



Source : Isolated from canned pumpkin. 



Habitat : Probably found in soil and 

 dust. 



Thermobacillus reductans Feirer (Soil 

 Sci., 23, 1927, 51) is said to resemble 

 Bacillus thermoindifferens except that 

 nitrites are formed from nitrates and the 

 minimum temperature is 40°C. 



Source: Two strains isolated from soil. 



Thermobacillus catenatus Feirer (Soil 

 Sci., 23, 1927, 53) may be related to this 

 group. The description is very incom- 

 plete. Its distinctive feature is the 

 production of indole. 



Source : Two strains isolated from soil. 



23. Bacillus thermodiastaticus Ber- 

 ge}- et al. (Type 1, Jiergey, Jour. Bact., 

 4, 1919, 304; Bergey et al.. Manual, 1st 

 ed., 1923, 310.) From Greek thermos, 

 hot and diaslalikos, separative; M. L., 

 enzjaiiatic, diastatic; hence diastatic at 

 high temperatures. 



Spores : Of less diameter than that of 

 the rods, ellipsoidal, central. 



Sporangia: Cj'lindrical. 



Rods: 0.5 to 0.7 by 2 to 3 microns, 



occurring in chains, with square ends. 

 Motile with peritrichous flagella. Gram- 

 positive. 



Gelatin stab : Liquefaction. 



Agar colonies : Grayish, spreading, with 

 lobate to funbriate borders. 



Agar slant: Growth thin, limited, 

 bluish-gray. 



Broth: Turbid. 



Litmus milk: Not coagulated, pep- 

 tonized. 



Potato: Growth slight, grayish. 



Nitrites produced from nitrates. 



Starch is hydrolyzed. 



Thermophilic, optimum temperature 

 65°C. No growth at 50°C. Growth at 

 75°C. 



Aerobic . 



Source: Isolated from dust and con- 

 taminated milk. 



Habitat : Probably widely distributed 

 in soil and dust. 



Thermobacillus diastasius Feirer (Soil 

 Sci., 23, 1927, 49) differs from Bacillus 

 thermodiastaticus only in that nitrites 

 are not formed from nitrates (Feirer). 



Source : Two strains isolated from soil. 



24. Bacillus cylindricus Blau. (Cent, 

 f. Bakt., II Abt., 15, 1905, 119.) From 

 Greek kylindrikos, cylindrical. 



Spores : Somewhat elongated, 0.7 to 1.1 

 by 1 .8 to 2.5 microns, terminal. Remnants 

 of sporangium adherent. Germination 

 equatorial and oblique. 



Sporangia : Cylindrical or only slightly 

 swollen at end, not in chains. 



Rods : On glucose agar at 60°C, 0.8 to 

 1.1 by 5.0 to 7.5 microns, occurring 

 singly and in pairs. Motile with peri- 

 trichous flagella. Cells store glycogen. 

 Gram-positive. 



Gelatin stab : No liquefaction. 



Glucose agar colonies : Grayish-white, 

 entire to lobed to dentate. By trans- 

 mitted light yellowish-brown centers 

 with brownish-yellow borders. Finely 

 fibrous structure. 



Glucose agar slant : Growth thin, dull, 

 grayish-white. 



Litmus milk : Unchanged. 



