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MANUAL OF DETERMINATIVE BACTERIOLOGY 



Potato : No growth. 



Nitrites not produced from nitrates. 



Starch not hydrolyzed. 



Thermophilic, optimum temperature 

 60°C to 70°C. 



Source : Isohxted from moist field soil in 

 Germany . 



Habitat : Probably found in dust and 

 soil. 



Bacillus calidus Blau (Cent. f. Bakt., 

 II Abt., 15, 1905, 134) differs so little from 

 the preceding species that it cannot be 

 considered as distinct. 



Source : Isolated from field soil in 

 Germany (Blau). Dust, ground feeds, 

 etc., about dairies and various dairy 

 products (Prickett, N. Y. Agr. Exp. Sta. 

 Tech. Bui. 147, 1928,45). 



25. Bacillus robustus Blau. (Cent. f. 

 Bakt., II Abt., 15, 1905, 126.) From 

 Latin robustus, oaken, hard, firm. 



Spores: Ellipsoidal, 1.0 by 1.6 to 2.2 

 microns, polar to medial. Remnants of 

 sporangium not adherent. Germination 

 prevailingly equatorial. 



Sporangia: Ellipsoidal to cylindrical, 

 not in chains. 



Rods: 1.0 to 1.2 by 3 to 4 microns, 

 occurring singly and in short chains. 

 Motile. Gram-positive. 



Glucose agar colonies : Circular, gray- 

 white. By transmitted light brownish- 

 yellow. Borders distinct, serrate to 

 lobed, finely granular. 



Glucose agar slant : Growth yellowish- 

 white, translucent, becoming grayish- 

 white, spreading, dull. 



Potato : Growth yellowish-whitc, moist, 

 glistening, smooth. 



Nitrites not produced from nitrates. 



Starch not hydrolyzed. 



Thermophilic, optimum temperature 

 55°C to 60°C. Grows at 65°C. 



Aerobic . 



Source : Isolated from field soil near a 

 forest in Germany. 



Habitat : Probably found in soil and 

 dust. 



Thermobacillus restatus Feirer (Soil 

 Sci., 23, 1927, 51) is said to correspond 



in some respects with Bacillus robustus. 

 Feirer states that it is not possible to 

 definitely establish the identity because 

 Blau failed to record the action of Ba- 

 cillus robustus on nitrates and several 

 other media and did not note the produc- 

 tion of H,S. 



Source : Three strains isolated from 

 soil. 



25a. Bacillus losanitchii Bergey et al. 

 {Bacillus thermophilus losanitchii 

 Georgevitch, Cent. f. Bakt., II Abt., 27, 

 1910, 164; Bergey et al., Manual, 1st ed., 

 1923, 313.) Named for Losanitch, near 

 Vranje. 



As far as can be determined from the 

 meager description, this organism does 

 not differ from Bacillus robustus except 

 perhaps as to the maximum temperature 

 allowing growth. Growth limits are 

 45°C to 78°C. 



Source: Isolated from water of hot 

 sulfur spring. Temperature of water 

 83°C. 



Habitat : Probably in natural hot 

 waters. 



Note: Georgevitch (Arch. f. Hyg., 72, 

 1910, 201) has described a thermophilic 

 aerobic spore-forming sulfur bacillus 

 from a hot sulfur spring at Vranje (Ser- 

 via) under the name Bacillus thermo- 

 philus vranjensis. This does not grow 

 on ordinary media unless sulfur com- 

 pounds are added. It has a tuft of flagella 

 at either end. Spores are ellipsoidal, 

 terminal, distend the rod, and show polar 

 germination. 



Georgevitch (Cent. f. Bakt., II Abt., 

 27, 1910, 150) describes a second thermo- 

 philic, motile, capsulated, ellipsoidal- 

 spored rod from a chalybeate hot spring 

 near Vranje under the name Bacillus 

 t h ermoph il us jivo in i . 



26. Bacillus calidolactis Hussong and 

 Hammer. (Jour. Baet., 15, 1928, 186.) 

 From Latin calidus, warm, hot and lac, 

 lactis, milk. 



Gorini states (R. 1st. Lombardo Sci. e 

 Lett. Rend., 76, 1942, 3) that Bacillus 



